OBJECTIVE: Blood/brain-glutamate grabbing is an emerging concept in the treatment of acute ischemic stroke, where essentially the deleterious effects of glutamate after ischemia are ameliorated by coaxing glutamate to enter the bloodstream and thus reducing its concentration in the brain. Aiming to demonstrate the clinical efficacy of blood glutamate grabbers in patients with stroke, in this study, we resorted to a drug-repositioning strategy for the discovery of new glutamate-grabbing drugs. METHODS: The glutamate-grabbing ability of 1,120 compounds (90% of which were drugs approved by the US Food and Drug Administration) was evaluated during an in vitro high-throughput screening campaign. Subsequently, the protective efficacy of the selected drugs was probed in an ischemic animal model and finally tested in stroke patients. RESULTS:Riboflavin (vitamin B2 ) was identified as the main hit compound. In ischemic animal models treated with riboflavin (1mg/kg), it was confirmed that blood glutamate reduction was associated with a significant reduction of infarct size. These results led to a randomized, double-blind, phase IIb clinical trial with patients with stroke. Fifty patients were randomized to 1 of the 2 study arms: the control group (placebo) and the experimental group (20mg of riboflavin [vitamin B2 Streuli@ ). Decrease in glutamate concentration was significantly greater (p < 0.029) in the treated group. Comparative analysis of the percentage improvement on the National Institutes of Health Stroke Scale score at discharge was slightly higher in the riboflavin-treated group than in the placebo group (33.7 ± 43.7 vs 48.9 ± 42.4%, p = 0.050). INTERPRETATION: This translational study represents the first human demonstration of the efficacy of blood glutamate grabbers in the treatment of patients with stroke, paving the way for the development of a promising novel protective therapy. Ann Neurol 2018;84:260-273.
RCT Entities:
OBJECTIVE: Blood/brain-glutamate grabbing is an emerging concept in the treatment of acute ischemic stroke, where essentially the deleterious effects of glutamate after ischemia are ameliorated by coaxing glutamate to enter the bloodstream and thus reducing its concentration in the brain. Aiming to demonstrate the clinical efficacy of blood glutamate grabbers in patients with stroke, in this study, we resorted to a drug-repositioning strategy for the discovery of new glutamate-grabbing drugs. METHODS: The glutamate-grabbing ability of 1,120 compounds (90% of which were drugs approved by the US Food and Drug Administration) was evaluated during an in vitro high-throughput screening campaign. Subsequently, the protective efficacy of the selected drugs was probed in an ischemic animal model and finally tested in strokepatients. RESULTS:Riboflavin (vitamin B2 ) was identified as the main hit compound. In ischemic animal models treated with riboflavin (1mg/kg), it was confirmed that blood glutamate reduction was associated with a significant reduction of infarct size. These results led to a randomized, double-blind, phase IIb clinical trial with patients with stroke. Fifty patients were randomized to 1 of the 2 study arms: the control group (placebo) and the experimental group (20mg of riboflavin [vitamin B2 Streuli@ ). Decrease in glutamate concentration was significantly greater (p < 0.029) in the treated group. Comparative analysis of the percentage improvement on the National Institutes of Health Stroke Scale score at discharge was slightly higher in the riboflavin-treated group than in the placebo group (33.7 ± 43.7 vs 48.9 ± 42.4%, p = 0.050). INTERPRETATION: This translational study represents the first human demonstration of the efficacy of blood glutamate grabbers in the treatment of patients with stroke, paving the way for the development of a promising novel protective therapy. Ann Neurol 2018;84:260-273.
Authors: Pablo Hervella; María Luz Alonso-Alonso; María Pérez-Mato; Manuel Rodríguez-Yáñez; Susana Arias-Rivas; Iria López-Dequidt; José M Pumar; Tomás Sobrino; Francisco Campos; José Castillo; Ramón Iglesias-Rey Journal: BMC Neurol Date: 2022-06-09 Impact factor: 2.903
Authors: R A Akasov; E V Khaydukov; D S Andreyuk; N V Sholina; A N Sheremeta; D V Romanov; G P Kostyuk; V Ya Panchenko; M V Kovalchuk Journal: Front Pharmacol Date: 2022-03-10 Impact factor: 5.810
Authors: Abbas Jarrahi; Molly Braun; Meenakshi Ahluwalia; Rohan V Gupta; Michael Wilson; Stephanie Munie; Pankaj Ahluwalia; John R Vender; Fernando L Vale; Krishnan M Dhandapani; Kumar Vaibhav Journal: Biomedicines Date: 2020-09-29
Authors: Ramón Iglesias-Rey; Andres da Silva-Candal; Manuel Rodríguez-Yáñez; Ana Estany-Gestal; Uxía Regueiro; Elena Maqueda; Paulo Ávila-Gómez; José Manuel Pumar; José Castillo; Tomás Sobrino; Francisco Campos; Pablo Hervella Journal: Transl Stroke Res Date: 2021-06-24 Impact factor: 6.829