| Literature DB >> 3000888 |
Abstract
A new restriction endonuclease, EcoO109, has been isolated from Escherichia coli H709c by polyethyleneimine (PEI) precipitation, DEAE-cellulose chromatography and heparin agarose chromatography. The yield was high, more than 3000 units/g of wet cells. The EcoO109 endonuclease recognizes and cleaves a nucleotide sequence of (formula: see text), in the presence of 10 mM Mg2+. The enzyme will be useful for structural analysis and molecular cloning of DNA because of the stability, high yield and easy handling of the producer strain.Entities:
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Year: 1985 PMID： 3000888 DOI： 10.1016/0378-1119(85)90192-1
Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688