Literature DB >> 3000832

cDNA clones coding for the beta-subunit of human liver alcohol dehydrogenase have differently sized 3'-non-coding regions.

L O Hedén, J O Höög, K Larsson, M Lake, E Lagerholm, A Holmgren, B L Vallee, H Jörnvall, H von Bahr-Lindström.   

Abstract

Three different size classes of cDNA clones coding for the beta 1-subunit of human alcohol dehydrogenase (ADH) were characterized from a human liver cDNA library. Clones were identified by hybridization with synthetic oligodeoxyribonucleotides. A total of 2530 nucleotides were determined, covering an ADH-coding region of 1122 nucleotides, a preceding 72-nucleotide segment and 3 types of 3'-non-coding region. The coding nucleotide sequence is in full agreement with the amino acid sequence of the beta 1-subunit. Of 8 clones identified, 6 had a short, 213-nucleotide 3'-non-coding region; 1 an intermediate, 590-nucleotide 3'-region; and 1 a long, 1330-nucleotide 3'-region. In addition, 2 unused polyadenylation signals were found. These results suggest that human liver beta-ADH mRNAs occur in several size classes, and that in addition to the consensus sequence AATAAA further signals are important for 3'-end formation.

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Year:  1986        PMID： 3000832     DOI： 10.1016/0014-5793(86)80111-9

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  6 in total

1.  Complete nucleotide and derived amino acid sequence of cDNA encoding the mitochondrial uncoupling protein of rat brown adipose tissue: lack of a mitochondrial targeting presequence.

Authors:  R G Ridley; H V Patel; G E Gerber; R C Morton; K B Freeman
Journal:  Nucleic Acids Res       Date:  1986-05-27       Impact factor: 16.971

2.  Precursor-product relationship between vitellogenin and the yolk proteins as derived from the complete sequence of a Xenopus vitellogenin gene.

Authors:  S Gerber-Huber; D Nardelli; J A Haefliger; D N Cooper; F Givel; J E Germond; J Engel; N M Green; W Wahli
Journal:  Nucleic Acids Res       Date:  1987-06-25       Impact factor: 16.971

3.  Species-specific differential cleavage and polyadenylation of plasminogen activator inhibitor type 1 hnRNA.

Authors:  P G Fattal; J J Billadello
Journal:  Nucleic Acids Res       Date:  1993-03-25       Impact factor: 16.971

4.  Regulation of gene expression of class I alcohol dehydrogenase by glucocorticoids.

Authors:  Y Dong; L Poellinger; S Okret; J O Höög; H von Bahr-Lindström; H Jörnvall; J A Gustafsson
Journal:  Proc Natl Acad Sci U S A       Date:  1988-02       Impact factor: 11.205

5.  Cloning and sequencing of cDNA encoding baboon liver alcohol dehydrogenase: evidence for a common ancestral lineage with the human alcohol dehydrogenase beta subunit and for class I ADH gene duplications predating primate radiation.

Authors:  A E Trezise; E A Godfrey; R S Holmes; I R Beacham
Journal:  Proc Natl Acad Sci U S A       Date:  1989-07       Impact factor: 11.205

6.  Multiple mRNAs for human alcohol dehydrogenase (ADH): developmental and tissue specific differences.

Authors:  V Bilanchone; G Duester; Y Edwards; M Smith
Journal:  Nucleic Acids Res       Date:  1986-05-12       Impact factor: 16.971
  6 in total

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