Background and Aims: Changes in the arrangement of cell wall components determine cell wall properties (integrity, stiffness), thereby affecting the macro-scale properties of fruits, which are important for consumers and industry. Arabinogalactan proteins (AGPs) are ubiquitous components of the plant cell, in which they have various functions. Currently, AGPs are considered to be one of the less well-known, enigmatic proteoglycans, a consequence of their heterogeneous structure and unclear mechanism of activity. Methods: An immunocytochemical study was conducted to elucidate the distribution of AGPs and pectic polysaccharides contained in apple (Malus × domestica) fruit during senescence. De-esterified homogalacturonan (LM19), methyl-esterified homogalacturonan (LM20), processed arabinan (LM16) and three AGP epitopes (JIM13, JIM15, MAC207) were identified in the fruit at three stages: fresh fruit, and fruit at 1 and 3 months of post-harvest storage. Key Results: Microscopy revealed spatio-temporal changes in the localization of all examined epitopes. Changes of fruit cell wall assembly and its degradation were confirmed by determination of the galacturonic acid content in the WSP (water soluble pectins), CSP (chelator soluble pectins) and DASP (dilute alkali soluble pectins) fractions. Conclusions: The results revealed dependencies between AGPs, arabinan and homogalacturonan distribution in apple fruit, which are correlated with changes in microstructure during senescence. We propose that AGPs are involved in establishment of the cell wall - plasma membrane continuum.
Background and Aims: Changes in the arrangement of cell wall components determine cell wall properties (integrity, stiffness), thereby affecting the macro-scale properties of fruits, which are important for consumers and industry. Arabinogalactan proteins (AGPs) are ubiquitous components of the plant cell, in which they have various functions. Currently, AGPs are considered to be one of the less well-known, enigmatic proteoglycans, a consequence of their heterogeneous structure and unclear mechanism of activity. Methods: An immunocytochemical study was conducted to elucidate the distribution of AGPs and pecticpolysaccharides contained in apple (Malus × domestica) fruit during senescence. De-esterified homogalacturonan (LM19), methyl-esterified homogalacturonan (LM20), processed arabinan (LM16) and three AGP epitopes (JIM13, JIM15, MAC207) were identified in the fruit at three stages: fresh fruit, and fruit at 1 and 3 months of post-harvest storage. Key Results: Microscopy revealed spatio-temporal changes in the localization of all examined epitopes. Changes of fruit cell wall assembly and its degradation were confirmed by determination of the galacturonic acid content in the WSP (water soluble pectins), CSP (chelator soluble pectins) and DASP (dilute alkali soluble pectins) fractions. Conclusions: The results revealed dependencies between AGPs, arabinan and homogalacturonan distribution in apple fruit, which are correlated with changes in microstructure during senescence. We propose that AGPs are involved in establishment of the cell wall - plasma membrane continuum.
Authors: Li Tan; Stefan Eberhard; Sivakumar Pattathil; Clayton Warder; John Glushka; Chunhua Yuan; Zhangying Hao; Xiang Zhu; Utku Avci; Jeffrey S Miller; David Baldwin; Charles Pham; Ronald Orlando; Alan Darvill; Michael G Hahn; Marcia J Kieliszewski; Debra Mohnen Journal: Plant Cell Date: 2013-01-31 Impact factor: 11.277
Authors: Shigeki Watanabe; Annedore Punge; Gunther Hollopeter; Katrin I Willig; Robert John Hobson; M Wayne Davis; Stefan W Hell; Erik M Jorgensen Journal: Nat Methods Date: 2010-11-21 Impact factor: 28.547
Authors: Maja G Rydahl; Stjepan K Krac Un; Jonatan U Fangel; Gurvan Michel; Alexia Guillouzo; Sabine Génicot; Jozef Mravec; Jesper Harholt; Casper Wilkens; Mohammed Saddik Motawia; Birte Svensson; Olivier Tranquet; Marie-Christine Ralet; Bodil Jørgensen; David S Domozych; William G T Willats Journal: Sci Rep Date: 2017-08-24 Impact factor: 4.379