D Maji1, M De La Fuente2, E Kucukal3, U D S Sekhon4, A H Schmaier5,6, A Sen Gupta4, U A Gurkan3,4, M T Nieman2, E X Stavrou7,6, P Mohseni1, M A Suster1. 1. Electrical Engineering and Computer Science Department, Case Western Reserve University, Cleveland, OH, USA. 2. Department of Pharmacology, Case Western Reserve University, Cleveland, OH, USA. 3. Mechanical and Aerospace Engineering Department, Case Western Reserve University, Cleveland, OH, USA. 4. Biomedical Engineering Department, Case Western Reserve University, Cleveland, OH, USA. 5. Department of Medicine, Hematology and Oncology Division, University Hospitals Cleveland Medical Center, Cleveland, OH, USA. 6. Department of Medicine, Hematology and Oncology Division, Case Western Reserve University School of Medicine, Cleveland, OH, USA. 7. Department of Medicine, Louis Stokes Veterans Administration Medical Center, Cleveland, OH, USA.
Abstract
Essentials ClotChip is a novel microsensor for comprehensive assessment of ex vivo hemostasis. Clinical samples show high sensitivity to detecting the entire hemostatic process. ClotChip readout exhibits distinct information on coagulation factor and platelet abnormalities. ClotChip has potential as a point-of-care platform for comprehensive hemostatic analysis. SUMMARY: Background Rapid point-of-care (POC) assessment of hemostasis is clinically important in patients with a variety of coagulation factor and platelet defects who have bleeding disorders. Objective To evaluate a novel dielectric microsensor, termed ClotChip, which is based on the electrical technique of dielectric spectroscopy for rapid, comprehensive assessment of whole blood coagulation. Methods The ClotChip is a three-dimensional, parallel-plate, capacitive sensor integrated into a single-use microfluidic channel with miniscule sample volume (< 10 μL). The ClotChip readout is defined as the temporal variation in the real part of dielectric permittivity of whole blood at 1 MHz. Results The ClotChip readout exhibits two distinct parameters, namely, the time to reach a permittivity peak (Tpeak ) and the maximum change in permittivity after the peak (Δεr,max ), which are, respectively, sensitive towards detecting non-cellular (i.e. coagulation factor) and cellular (i.e. platelet) abnormalities in the hemostatic process. We evaluated the performance of ClotChip using clinical blood samples from 15 healthy volunteers and 12 patients suffering from coagulation defects. The ClotChip Tpeak parameter exhibited superior sensitivity at distinguishing coagulation disorders as compared with conventional screening coagulation tests. Moreover, the ClotChip Δεr,max parameter detected platelet function inhibition induced by aspirin and exhibited strong positive correlation with light transmission aggregometry. Conclusions This study demonstrates that ClotChip assesses multiple aspects of the hemostatic process in whole blood on a single disposable cartridge, highlighting its potential as a POC platform for rapid, comprehensive hemostatic analysis.
Essentials ClotChip is a novel microsensor for comprehensive assessment of ex vivo hemostasis. Clinical samples show high sensitivity to detecting the entire hemostatic process. ClotChip readout exhibits distinct information on coagulation factor and platelet abnormalities. ClotChip has potential as a point-of-care platform for comprehensive hemostatic analysis. SUMMARY: Background Rapid point-of-care (POC) assessment of hemostasis is clinically important in patients with a variety of coagulation factor and platelet defects who have bleeding disorders. Objective To evaluate a novel dielectric microsensor, termed ClotChip, which is based on the electrical technique of dielectric spectroscopy for rapid, comprehensive assessment of whole blood coagulation. Methods The ClotChip is a three-dimensional, parallel-plate, capacitive sensor integrated into a single-use microfluidic channel with miniscule sample volume (< 10 μL). The ClotChip readout is defined as the temporal variation in the real part of dielectric permittivity of whole blood at 1 MHz. Results The ClotChip readout exhibits two distinct parameters, namely, the time to reach a permittivity peak (Tpeak ) and the maximum change in permittivity after the peak (Δεr,max ), which are, respectively, sensitive towards detecting non-cellular (i.e. coagulation factor) and cellular (i.e. platelet) abnormalities in the hemostatic process. We evaluated the performance of ClotChip using clinical blood samples from 15 healthy volunteers and 12 patients suffering from coagulation defects. The ClotChip Tpeak parameter exhibited superior sensitivity at distinguishing coagulation disorders as compared with conventional screening coagulation tests. Moreover, the ClotChip Δεr,max parameter detected platelet function inhibition induced by aspirin and exhibited strong positive correlation with light transmission aggregometry. Conclusions This study demonstrates that ClotChip assesses multiple aspects of the hemostatic process in whole blood on a single disposable cartridge, highlighting its potential as a POC platform for rapid, comprehensive hemostatic analysis.
Authors: Debnath Maji; Michael A Suster; Erdem Kucukal; Ujjal D S Sekhon; Anirban Sen Gupta; Umut A Gurkan; Evi X Stavrou; Pedram Mohseni Journal: IEEE Trans Biomed Circuits Syst Date: 2017-09-12 Impact factor: 3.833
Authors: James R Byrnes; Cédric Duval; Yiming Wang; Caroline E Hansen; Byungwook Ahn; Micah J Mooberry; Martha A Clark; Jill M Johnsen; Susan T Lord; Wilbur A Lam; Joost C M Meijers; Heyu Ni; Robert A S Ariëns; Alisa S Wolberg Journal: Blood Date: 2015-08-31 Impact factor: 22.113
Authors: Yuncheng Man; Debnath Maji; Ran An; Sanjay P Ahuja; Jane A Little; Michael A Suster; Pedram Mohseni; Umut A Gurkan Journal: Lab Chip Date: 2021-03-05 Impact factor: 6.799
Authors: Brady Trevisan; Alshaimaa Morsi; Julio Aleman; Martin Rodriguez; Jordan Shields; Diane Meares; Andrew M Farland; Christopher B Doering; H Trent Spencer; Anthony Atala; Aleks Skardal; Christopher D Porada; Graça Almeida-Porada Journal: Front Bioeng Biotechnol Date: 2021-03-01
Authors: Debnath Maji; Aman Opneja; Michael A Suster; Kara L Bane; Brigid M Wilson; Pedram Mohseni; Evi X Stavrou Journal: Thromb Haemost Date: 2020-09-02 Impact factor: 5.249