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Literature DB >> 30002272

Fluorescence spectroscopy for revealing mechanisms in biology: Strengths and pitfalls.

Abstract

This article describes the basic principles of steady-state and time-resolved fluorescence. The formal equivalence of the two methodologies is described first, followed by the extra advantages of time-resolved methods in revealing population heterogeneity in complex systems encountered in biology. Several examples from the author's work are described in support of the above contention. Finally, several misinterpretations and pitfalls in the interpretation of fluorescence data and their remedy are described.

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Year: 2018 PMID： 30002272

Source DB: PubMed Journal: J Biosci ISSN： 0250-5991 Impact factor: 1.826

42 in total

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4. Oligomerization of the serotonin(1A) receptor in live cells: a time-resolved fluorescence anisotropy approach.

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Review 5. Fluorescence spectroscopy in molecular description of biological processes.

Authors: G Krishnamoorthy
Journal: Indian J Biochem Biophys Date: 2003-06 Impact factor: 1.918

6. Fully integrated silicon probes for high-density recording of neural activity.

Authors: James J Jun; Nicholas A Steinmetz; Joshua H Siegle; Daniel J Denman; Marius Bauza; Brian Barbarits; Albert K Lee; Costas A Anastassiou; Alexandru Andrei; Çağatay Aydın; Mladen Barbic; Timothy J Blanche; Vincent Bonin; João Couto; Barundeb Dutta; Sergey L Gratiy; Diego A Gutnisky; Michael Häusser; Bill Karsh; Peter Ledochowitsch; Carolina Mora Lopez; Catalin Mitelut; Silke Musa; Michael Okun; Marius Pachitariu; Jan Putzeys; P Dylan Rich; Cyrille Rossant; Wei-Lung Sun; Karel Svoboda; Matteo Carandini; Kenneth D Harris; Christof Koch; John O'Keefe; Timothy D Harris
Journal: Nature Date: 2017-11-08 Impact factor: 49.962

7. Cell type and spatial location dependence of cytoplasmic viscosity measured by time-resolved fluorescence microscopy.

Authors: A Srivastava; G Krishnamoorthy
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8. Maximum entropy method of data analysis in time-resolved spectroscopy.

Authors: J C Brochon
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9. SNARF-1 as an intracellular pH indicator in laser microspectrofluorometry: a critical assessment.

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10. p53 amyloid formation leading to its loss of function: implications in cancer pathogenesis.

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5 in total

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Review 2. Site-Directed Fluorescence Approaches for Dynamic Structural Biology of Membrane Peptides and Proteins.

Authors: H Raghuraman; Satyaki Chatterjee; Anindita Das
Journal: Front Mol Biosci Date: 2019-09-25

3. Site-directed fluorescence approaches to monitor the structural dynamics of proteins using intrinsic Trp and labeled with extrinsic fluorophores.

Authors: Rupasree Brahma; Anindita Das; H Raghuraman
Journal: STAR Protoc Date: 2022-02-28

4. Characterization of Two NMN Deamidase Mutants as Possible Probes for an NMN Biosensor.

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Journal: Int J Mol Sci Date: 2021-06-13 Impact factor: 5.923

5. Experimental approach to study the effect of mutations on the protein folding pathway.

Authors: Elena V Nemtseva; Marina A Gerasimova; Tatiana N Melnik; Bogdan S Melnik
Journal: PLoS One Date: 2019-01-14 Impact factor: 3.240

5 in total