OBJECTIVES: B7 family has been identified as co-stimulatory or co-inhibitory molecules on T-cell response and plays an important role in tumour mortality and malignancy. In this study, the expression pattern of B7 family in gastrointestinal (GI) cancer was examined. Its upstream regulating mechanism, downstream targets and association with clinical parameters were also studied. MATERIALS AND METHODS: The expression level of B7 members was analysed by FIREHOUSE. The gene mutation, DNA methylation, association with clinical parameters and downstream network of B7 members were analysed in cBioportal. The mutation frequency was analysed by Catalogue of Somatic Mutations in Cancer (COSMIC) analysis. The phylogenetic tree was constructed in MEGA7. The interaction protein domain analysis was performed by Pfam 31.0. RESULTS: Differential expression of B7 family molecules was detected in different kinds of GI cancer. High-frequency gene alteration was found in tumour samples. There was negative correlation of promoter methylation and mRNA expression of B7 family members in tumour samples, suggesting the epigenetic basis of B7 family gene deregulation in GI cancer. The overexpression of B7-H1 in pancreatic cancer, B7-H5 in oesophageal cancer and B7-H6 in liver cancer were significantly associated with worse overall survival. Finally, by network analysis, we identified some potential interacting proteins for B7-1/2 and B7-H1/DC. CONCLUSIONS: Overall, our study suggested that B7 member deregulation was strongly involved in GI cancer tumorigenesis.
OBJECTIVES: B7 family has been identified as co-stimulatory or co-inhibitory molecules on T-cell response and plays an important role in tumour mortality and malignancy. In this study, the expression pattern of B7 family in gastrointestinal (GI) cancer was examined. Its upstream regulating mechanism, downstream targets and association with clinical parameters were also studied. MATERIALS AND METHODS: The expression level of B7 members was analysed by FIREHOUSE. The gene mutation, DNA methylation, association with clinical parameters and downstream network of B7 members were analysed in cBioportal. The mutation frequency was analysed by Catalogue of Somatic Mutations in Cancer (COSMIC) analysis. The phylogenetic tree was constructed in MEGA7. The interaction protein domain analysis was performed by Pfam 31.0. RESULTS: Differential expression of B7 family molecules was detected in different kinds of GI cancer. High-frequency gene alteration was found in tumour samples. There was negative correlation of promoter methylation and mRNA expression of B7 family members in tumour samples, suggesting the epigenetic basis of B7 family gene deregulation in GI cancer. The overexpression of B7-H1 in pancreatic cancer, B7-H5 in oesophageal cancer and B7-H6 in liver cancer were significantly associated with worse overall survival. Finally, by network analysis, we identified some potential interacting proteins for B7-1/2 and B7-H1/DC. CONCLUSIONS: Overall, our study suggested that B7 member deregulation was strongly involved in GI cancer tumorigenesis.
Authors: Yuping Sun; Yunshan Wang; Jianqiang Zhao; Ming Gu; Ricardo Giscombe; Ann Kari Lefvert; Xiongbiao Wang Journal: Lung Cancer Date: 2006-06-19 Impact factor: 5.705
Authors: Dong Ju Son; Jie Zheng; Yu Yeon Jung; Chul Ju Hwang; Hee Pom Lee; Ju Rang Woo; Song Yi Baek; Young Wan Ham; Min Woong Kang; Minho Shong; Gi Ryang Kweon; Min Jong Song; Jae Kyung Jung; Sang-Bae Han; Bo Yeon Kim; Do Young Yoon; Bu Young Choi; Jin Tae Hong Journal: Theranostics Date: 2017-10-16 Impact factor: 11.556
Authors: Michiel C Mommersteeg; Bing Ting Yu; Thierry P P van den Bosch; Johan H von der Thüsen; Ernst J Kuipers; Michael Doukas; Manon C W Spaander; Maikel P Peppelenbosch; Gwenny M Fuhler Journal: Helicobacter Date: 2022-07-28 Impact factor: 5.182