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Literature DB >> 2999082

lac Up-promoter mutants with increased homology to the consensus promoter sequence.

W Mandecki, R A Goldman, B S Powell, M H Caruthers.

Abstract

Four lac promoter mutants were constructed. The mutations increased the homology between the lac promoter and the consensus promoter sequences by introducing the consensus -10 and -35 regions and the consensus spacing of 17 residues between these two regions. The promoter mutants were cloned into a pBR322-derivatized vector upstream from the lacZ gene, and levels of beta-galactosidase were an indication of promoter activity. All mutants exhibited higher activity than did the wild-type promoter.

Entities: Gene

Mesh：

Substances：
DNA Restriction Enzymes

Year: 1985 PMID： 2999082 PMCID： PMC219337 DOI： 10.1128/jb.164.3.1353-1355.1985

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

15 in total

Review 1. Regulatory sequences involved in the promotion and termination of RNA transcription.

Authors: M Rosenberg; D Court
Journal: Annu Rev Genet Date: 1979 Impact factor: 16.830

2. A lac promoter with a changed distance between -10 and -35 regions.

Authors: W Mandecki; W S Reznikoff
Journal: Nucleic Acids Res Date: 1982-02-11 Impact factor: 16.971

Review 3. Compilation and analysis of Escherichia coli promoter DNA sequences.

Authors: D K Hawley; W R McClure
Journal: Nucleic Acids Res Date: 1983-04-25 Impact factor: 16.971

4. Spacer mutations in the lac ps promoter.

Authors: J E Stefano; J D Gralla
Journal: Proc Natl Acad Sci U S A Date: 1982-02 Impact factor: 11.205

Review 5. E. coli RNA polymerase interacts homologously with two different promoters.

Authors: U Siebenlist; R B Simpson; W Gilbert
Journal: Cell Date: 1980-06 Impact factor: 41.582

6. Sequencing end-labeled DNA with base-specific chemical cleavages.

Authors: A M Maxam; W Gilbert
Journal: Methods Enzymol Date: 1980 Impact factor: 1.600

7. Catabolite-insensitive revertants of lac promoter mutants.

Authors: A E Silverstone; R R Arditti; B Magasanik
Journal: Proc Natl Acad Sci U S A Date: 1970-07 Impact factor: 11.205

8. The tac promoter: a functional hybrid derived from the trp and lac promoters.

Authors: H A de Boer; L J Comstock; M Vasser
Journal: Proc Natl Acad Sci U S A Date: 1983-01 Impact factor: 11.205

9. Chemical synthesis of a gene encoding the human complement fragment C5a and its expression in Escherichia coli.

Authors: W Mandecki; K W Mollison; T J Bolling; B S Powell; G W Carter; J L Fox
Journal: Proc Natl Acad Sci U S A Date: 1985-06 Impact factor: 11.205

10. Chemical synthesis and biochemical reactivity of bacteriophage lambda PR promoter.

Authors: P L deHaseth; R A Goldman; C L Cech; M H Caruthers
Journal: Nucleic Acids Res Date: 1983-02-11 Impact factor: 16.971

10 in total

1. Hierarchies of base pair preferences in the P22 ant promoter.

Authors: H Moyle; C Waldburger; M M Susskind
Journal: J Bacteriol Date: 1991-03 Impact factor: 3.490

2. Mutation of the promoter and LexA binding sites of cea, the gene encoding colicin E1.

Authors: B Salles; G M Weinstock
Journal: Mol Gen Genet Date: 1989-02

3. Nucleotide sequence of the kanamycin resistance determinant of the pneumococcal transposon Tn1545: evolutionary relationships and transcriptional analysis of aphA-3 genes.

Authors: F Caillaud; P Trieu-Cuot; C Carlier; P Courvalin
Journal: Mol Gen Genet Date: 1987-05

lac Up-promoter mutants with increased homology to the consensus promoter sequence.

Review 1. Regulatory sequences involved in the promotion and termination of RNA transcription.

2. A lac promoter with a changed distance between -10 and -35 regions.

Review 3. Compilation and analysis of Escherichia coli promoter DNA sequences.

4. Spacer mutations in the lac ps promoter.

Review 5. E. coli RNA polymerase interacts homologously with two different promoters.

6. Sequencing end-labeled DNA with base-specific chemical cleavages.

7. Catabolite-insensitive revertants of lac promoter mutants.

8. The tac promoter: a functional hybrid derived from the trp and lac promoters.

9. Chemical synthesis of a gene encoding the human complement fragment C5a and its expression in Escherichia coli.

10. Chemical synthesis and biochemical reactivity of bacteriophage lambda PR promoter.

1. Hierarchies of base pair preferences in the P22 ant promoter.

2. Mutation of the promoter and LexA binding sites of cea, the gene encoding colicin E1.

3. Nucleotide sequence of the kanamycin resistance determinant of the pneumococcal transposon Tn1545: evolutionary relationships and transcriptional analysis of aphA-3 genes.

4. The effects of mutations in the ant promoter of phage P22 depend on context.

5. Efficiency of transcription from promoter sequence variants in Lactobacillus is both strain and context dependent.

6. Mutational analysis of the lac regulatory region: second-site changes that activate mutant promoters.

7. Mutations that affect activity of the Rhizobium meliloti trpE(G) promoter in Rhizobium meliloti and Escherichia coli.

8. Saturation mutagenesis of an Escherichia coli rRNA promoter and initial characterization of promoter variants.

9. Mutations of the Escherichia coli lacUV5 promoter resulting in increased expression in Bacillus subtilis.

10. Pyruvate kinase-deficient Escherichia coli exhibits increased plasmid copy number and cyclic AMP levels.