| Literature DB >> 29988095 |
Francesca Pennacchietti1, Ekaterina O Serebrovskaya2, Aline R Faro3, Irina I Shemyakina2, Nina G Bozhanova2, Alexey A Kotlobay2, Nadya G Gurskaya2, Andreas Bodén1, Jes Dreier1, Dmitry M Chudakov2, Konstantin A Lukyanov2, Vladislav V Verkhusha3, Alexander S Mishin4, Ilaria Testa5.
Abstract
Reversibly photoswitchable fluorescent proteins (rsFPs) are gaining popularity as tags for optical nanoscopy because they make it possible to image with lower light doses. However, green rsFPs need violet-blue light for photoswitching, which is potentially phototoxic and highly scattering. We developed new rsFPs based on FusionRed that are reversibly photoswitchable with green-orange light. The rsFusionReds are bright and exhibit rapid photoswitching, thereby enabling nanoscale imaging of living cells.Mesh：
Substances：
Year: 2018 PMID： 29988095 DOI： 10.1038/s41592-018-0052-9
Source DB: PubMed Journal: Nat Methods ISSN： 1548-7091 Impact factor: 28.547