| Literature DB >> 2998769 |
M J Allday, J H Sinclair, A J MacGillivray, J H Sang.
Abstract
In a search for exogenous promoters which function in cultured Drosophila cells, we have co-transfected a D. melanogaster cell line with an Epstein-Barr virus (EBV) cosmid clone which encodes the Epstein-Barr nuclear antigen (EBNA-1). Here we report that Drosophila cells containing stably integrated copies of EBNA-1 encoding DNA synthesise a polypeptide of mol. wt. identical to that of authentic EBNA-1, which is detectable with EBNA-positive but not EBNA-negative human serum. As in EBV-transformed lymphoblastoid cells, this neo-antigen is associated with the nucleus of transfected cells suggesting that cellular localisation signals which operate in mammalian cells are also recognised in insect cells.Entities:
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Year: 1985 PMID： 2998769 PMCID： PMC554604 DOI： 10.1002/j.1460-2075.1985.tb04029.x
Source DB: PubMed Journal: EMBO J ISSN： 0261-4189 Impact factor: 11.598