Literature DB >> 2998353

cys154 Is important for lac permease activity in Escherichia coli.

D R Menick, H K Sarkar, M S Poonian, H R Kaback.   

Abstract

The lac Y gene of Escherichia coli which encodes the lac permease has been modified by oligonucleotide-directed, site-specific mutagenesis such that cys154 is replaced with either gly or ser. Permease with gly in place of cys154 exhibits essentially no transport activity, while substitution of cys154 with ser also causes marked, though less complete loss of activity. The findings suggest that cys154 plays an important role in lactose:H+ symport.

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Year:  1985        PMID： 2998353     DOI： 10.1016/0006-291x(85)91002-2

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  25 in total

1.  Sugar binding induces the same global conformational change in purified LacY as in the native bacterial membrane.

Authors:  Yiling Nie; H Ronald Kaback
Journal:  Proc Natl Acad Sci U S A       Date:  2010-05-10       Impact factor: 11.205

Review 2.  Energy coupling mechanisms of MFS transporters.

Authors:  Xuejun C Zhang; Yan Zhao; Jie Heng; Daohua Jiang
Journal:  Protein Sci       Date:  2015-09-18       Impact factor: 6.725

Review 3.  Lessons from lactose permease.

Authors:  Lan Guan; H Ronald Kaback
Journal:  Annu Rev Biophys Biomol Struct       Date:  2006

4.  Energetics of ligand-induced conformational flexibility in the lactose permease of Escherichia coli.

Authors:  Yiling Nie; Irina Smirnova; Vladimir Kasho; H Ronald Kaback
Journal:  J Biol Chem       Date:  2006-09-26       Impact factor: 5.157

5.  Single-molecule FRET reveals sugar-induced conformational dynamics in LacY.

Authors:  Devdoot S Majumdar; Irina Smirnova; Vladimir Kasho; Eyal Nir; Xiangxu Kong; Shimon Weiss; H Ronald Kaback
Journal:  Proc Natl Acad Sci U S A       Date:  2007-05-14       Impact factor: 11.205

6.  Genetic selection for a highly functional cysteine-less membrane protein using site saturation mutagenesis.

Authors:  Cassandra S Arendt; Keirei Ri; Phillip A Yates; Buddy Ullman
Journal:  Anal Biochem       Date:  2007-03-30       Impact factor: 3.365

7.  Electrophysiological characterization of LacY.

Authors:  Juan J Garcia-Celma; Irina N Smirnova; H Ronald Kaback; Klaus Fendler
Journal:  Proc Natl Acad Sci U S A       Date:  2009-04-21       Impact factor: 11.205

8.  Evolutionary mix-and-match with MFS transporters II.

Authors:  M Gregor Madej; H Ronald Kaback
Journal:  Proc Natl Acad Sci U S A       Date:  2013-11-20       Impact factor: 11.205

9.  Apo-intermediate in the transport cycle of lactose permease (LacY).

Authors:  M Gregor Madej; Sonya N Soro; H Ronald Kaback
Journal:  Proc Natl Acad Sci U S A       Date:  2012-09-24       Impact factor: 11.205

10.  Nucleotide sequences and operon structure of plasmid-borne genes mediating uptake and utilization of raffinose in Escherichia coli.

Authors:  C Aslanidis; K Schmid; R Schmitt
Journal:  J Bacteriol       Date:  1989-12       Impact factor: 3.490
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