AIM: To investigate the regulation of lipoxygenase (LOX)-1 and Dectin-1 on interleukin-10 (IL-10) production in mice with Aspergillus fumigatus (A. fumigatus) keratitis. METHODS: The corneas of C57BL/6 mice were pretreated with LOX-1 inhibitor Poly(I) or Dectin-1 siRNA separately before the infection of A. fumigatus. Polymerase chain reaction (PCR) and Western blot were used to detect the expression of IL-10. RESULTS: The mRNA and protein expressions of IL-10 were significantly increased in mice with A. fumigatus keratitis. Compared with the group pretreated with sterile water before infection, Poly(I) pretreatment suppressed IL-10 expression significantly. Compared with the group pretreated with scrambled siRNA before infection, Dectin-1 siRNA pretreatment significantly reduced IL-10 expression in response to A. fumigatus infection. CONCLUSION: LOX-1 and Dectin-1 regulate IL-10 production in mouse A. fumigatus keratitis.
AIM: To investigate the regulation of lipoxygenase (LOX)-1 and Dectin-1 on interleukin-10 (IL-10) production in mice with Aspergillus fumigatus (A. fumigatus) keratitis. METHODS: The corneas of C57BL/6 mice were pretreated with LOX-1 inhibitor Poly(I) or Dectin-1 siRNA separately before the infection of A. fumigatus. Polymerase chain reaction (PCR) and Western blot were used to detect the expression of IL-10. RESULTS: The mRNA and protein expressions of IL-10 were significantly increased in mice with A. fumigatuskeratitis. Compared with the group pretreated with sterile water before infection, Poly(I) pretreatment suppressed IL-10 expression significantly. Compared with the group pretreated with scrambled siRNA before infection, Dectin-1 siRNA pretreatment significantly reduced IL-10 expression in response to A. fumigatus infection. CONCLUSION:LOX-1 and Dectin-1 regulate IL-10 production in mouseA. fumigatuskeratitis.
Authors: Neil C Rogers; Emma C Slack; Alexander D Edwards; Martijn A Nolte; Oliver Schulz; Edina Schweighoffer; David L Williams; Siamon Gordon; Victor L Tybulewicz; Gordon D Brown; Caetano Reis e Sousa Journal: Immunity Date: 2005-04 Impact factor: 31.745
Authors: Maria Teixeira-Coelho; Joana Guedes; Pedro Ferreirinha; Ashleigh Howes; Jorge Pedrosa; Fernando Rodrigues; Wi S Lai; Perry J Blackshear; Anne O'Garra; António G Castro; Margarida Saraiva Journal: Eur J Immunol Date: 2013-12-16 Impact factor: 5.532