Hideyuki Wada1,2, Hoon Hyun3, Kai Bao1, Jeong Heon Lee1, Georges El Fakhri1, Yongdoo Choi4, Hak Soo Choi1. 1. Gordon Center for Medical Imaging, Department of Radiology, Massachusetts General Hospital and Harvard Medical School, Boston, MA 02114, USA. 2. Department of Gastroenterological Surgery II, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan. 3. Department of Biomedical Science, Chonnam National University Medical School, Gwangju 501-746, South Korea. 4. Biomarker Branch, National Cancer Center, 323 Ilsan-ro, Goyang, Gyeonggi 10408, South Korea.
Abstract
Lymphadenectomy is a prerequisite for most malignancies to define the precise staging of cancer, as well as resect the possible metastases completely. While it improves prognosis, lymphadenectomy often causes postoperative edema or bleeding because of unclear surgical margins. In this study, we synthesized near-infrared (NIR) fluorescent nanoprobes with conjugating various mannose moieties on the surface to target macrophages in the lymph node. Armed with these NIR nanoprobes, we demonstrated the feasibility of intraoperative pan lymph nodes (PLN) mapping and real-time optical imaging under the NIR fluorescence imaging system. We found that even single mannose-conjugated ZW800-1 showed specific uptake in lymph nodes within 4 h, and multiple mannose-employed polyrotaxanes highlighted PLN efficiently with low background signals in major organs. This technology can help surgeons perform lymphadenectomy with ease and safety by identifying all regional lymph nodes proficiently after a single intravenous injection of NIR nanoprobes.
Lymphadenectomy is a prerequisite for most malignancies to define the precise staging of pan class="Disease">cancer, as well as resect the possible metastases completely. While it improves prognosis, lymphadenectomy often causes postoperative edema or bleeding because of unclear surgical margins. In this study, we synthesized near-infrared (NIR) fluorescent nanoprobes with conjugating various mannose moieties on the surface to target macrophages in the lymph node. Armed with these NIR nanoprobes, we demonstrated the feasibility of intraoperative pan lymph nodes (PLN) mapping and real-time optical imaging under the NIR fluorescence imaging system. We found that even single mannose-conjugated ZW800-1 showed specific uptake in lymph nodes within 4 h, and multiple mannose-employed polyrotaxanes highlighted PLN efficiently with low background signals in major organs. This technology can help surgeons perform lymphadenectomy with ease and safety by identifying all regional lymph nodes proficiently after a single intravenous injection of NIR nanoprobes.
Authors: Hoon Hyun; Mark W Bordo; Khaled Nasr; Dina Feith; Jeong Heon Lee; Soon Hee Kim; Yoshitomo Ashitate; Lorissa A Moffitt; Mireille Rosenberg; Maged Henary; Hak Soo Choi; John V Frangioni Journal: Contrast Media Mol Imaging Date: 2012 Nov-Dec Impact factor: 3.161