| Literature DB >> 29953890 |
Wei Wang1, Fenmin Zhang2, Qian Li1, Hui Chen3, Weijie Zhang1, Ping Yu4, Ting Zhao4, Guanghua Mao3, Weiwei Feng3, Liuqing Yang5, Xiangyang Wu6.
Abstract
The structural characterization, antioxidant activity and protective effect against H2O2-induced injury RAW264.7 cells of a new polysaccharide (MP1) isolated from Lepidium meyenii Walp. were investigated. The molecular weight was estimated to be 4.67 × 105 Da by HPLC-ELSD analysis. Monosaccharide composition was determined to be arabinose and galactose in a molar ratio of 2:1 by GC-MS and PMP-HPLC-UV analysis. Methylation combined with 1D and 2D NMR spectrum analysis revealed that MP1 was an arabinogalactan. MP1 possessed a moderate antioxidant activity in vitro in DPPH, ABTS, superoxide and hydroxyl radicals scavenging, Fe2+ chelating, lipid peroxidation inhibition and reducing power assays. MP1 could also effectively protect RAW264.7 cells from H2O2-induced injury by maintaining stable cell viability, decreasing ROS, MDA and LDH level and enhancing SOD, GSH-Px and CAT activity. These results suggested the potential utilization of MP1 as a natural antioxidant for food and pharmaceutical applications.Entities:
Keywords: Antioxidant activity; Lepidium meyenii Walp.; NMR; Polysaccharide; Structure characterization
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Year: 2018 PMID： 29953890 DOI： 10.1016/j.ijbiomac.2018.06.117
Source DB: PubMed Journal: Int J Biol Macromol ISSN： 0141-8130 Impact factor: 6.953