Literature DB >> 29951571

Purification of Total RNA from DSS-treated Murine Tissue via Lithium Chloride Precipitation.

Emilie Viennois1, Anika Tahsin1, Didier Merlin1,2.   

Abstract

We have developed a protocol to purify RNA from DSS (Dextran Sulfate Sodium)-treated mouse tissues. This method, which prevents downstream inhibition of q-RT-PCR observed in DSS-treated tissues, relies on successive precipitations with lithium chloride.

Entities:  

Keywords:  Colitis; Dextran sodium sulfate; Lithium chloride; RNA; q-RT-PCR

Year:  2018        PMID： 29951571      PMCID： PMC6017984          DOI： 10.21769/BioProtoc.2829

Source DB:  PubMed          Journal:  Bio Protoc        ISSN： 2331-8325


  5 in total

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2.  A method for isolation of intact, translationally active ribonucleic acid.

Authors:  G Cathala; J F Savouret; B Mendez; B L West; M Karin; J A Martial; J D Baxter
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Journal:  Pharmacol Res       Date:  2016-03-09       Impact factor: 7.658

4.  Dextran sodium sulfate inhibits the activities of both polymerase and reverse transcriptase: lithium chloride purification, a rapid and efficient technique to purify RNA.

Authors:  Emilie Viennois; Fengyuan Chen; Hamed Laroui; Mark T Baker; Didier Merlin
Journal:  BMC Res Notes       Date:  2013-09-08

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  5 in total
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Authors:  Robin C Su; Thomas M Blomquist; Andrew L Kleinhenz; Fatimah K Khalaf; Prabhatchandra Dube; Apurva Lad; Joshua D Breidenbach; Chrysan J Mohammed; Shungang Zhang; Caitlin E Baum; Deepak Malhotra; David J Kennedy; Steven T Haller
Journal:  Toxins (Basel)       Date:  2019-06-25       Impact factor: 4.546

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7.  Dairy Propionibacterium freudenreichii ameliorates acute colitis by stimulating MUC2 expression in intestinal goblet cell in a DSS-induced colitis rat model.

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  7 in total

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