Literature DB >> 6198133

A method for isolation of intact, translationally active ribonucleic acid.

G Cathala, J F Savouret, B Mendez, B L West, M Karin, J A Martial, J D Baxter.   

Abstract

A method for isolation of large, translationally active RNA species is presented. The procedure involves homogenization of cells or tissues in 5 M guanidine monothiocyanate followed by direct precipitation of RNA from the guanidinium by 4 M LiCl. Modifications are described for use with tissue culture cells, yeast, tissues, or isolated nuclei. The advantages of the procedure include speed, simplicity, avoidance of an ultracentrifugation, and its applicability to large numbers of small samples. The procedure yields large mRNA precursors up to 10 kb and mRNA species which translate very well. However, small (less than 300 nucleotides) RNA species are recovered with a poor yield.

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Year:  1983        PMID： 6198133     DOI： 10.1089/dna.1983.2.329

Source DB:  PubMed          Journal:  DNA        ISSN： 0198-0238


  394 in total

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2.  Tissue-specific expression from a compound TATA-dependent and TATA-independent promoter.

Authors:  P A Garrity; B J Wold
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3.  Isolation of pathogen/stress-inducible cDNAs from alfalfa by mRNA differential display.

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Journal:  Plant Mol Biol       Date:  1997-03       Impact factor: 4.076

4.  Differential Expression of the Two Subunits of Tomato Polygalacturonase Isoenzyme 1 in Wild-Type and rin Tomato Fruit.

Authors:  L. Zheng; C. F. Watson; D. DellaPenna
Journal:  Plant Physiol       Date:  1994-08       Impact factor: 8.340

5.  Characterization of the Early Stages of Genetic Salt-Stress Responses in Salt-Tolerant Lophopyrum elongatum, Salt-Sensitive Wheat, and Their Amphiploid.

Authors:  A. F. Galvez; P. J. Gulick; J. Dvorak
Journal:  Plant Physiol       Date:  1993-09       Impact factor: 8.340

6.  Erythropoietin changes the globin program of an interleukin 3-dependent multipotential cell line.

Authors:  T Enver; B Nakamoto; J Karlinsey; B Josephson; J Greenberger; T Papayannopoulou
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7.  Disruption of the cyclosporin synthetase gene of Tolypocladium niveum.

Authors:  G Weber; E Leitner
Journal:  Curr Genet       Date:  1994 Nov-Dec       Impact factor: 3.886

8.  Induction of glucose oxidase, catalase, and lactonase in Aspergillus niger.

Authors:  F B Witteveen; P J van de Vondervoort; H C van den Broeck; A C van Engelenburg; L H de Graaff; M H Hillebrand; P J Schaap; J Visser
Journal:  Curr Genet       Date:  1993-11       Impact factor: 3.886

9.  Coordinate Gene Response to Salt Stress in Lophopyrum elongatum.

Authors:  P J Gulick; J Dvorák
Journal:  Plant Physiol       Date:  1992-11       Impact factor: 8.340

10.  Genomic structure of human microtubule-associated protein 2 (MAP-2) and characterization of additional MAP-2 isoforms.

Authors:  N Kalcheva; J Albala; K O'Guin; H Rubino; C Garner; B Shafit-Zagardo
Journal:  Proc Natl Acad Sci U S A       Date:  1995-11-21       Impact factor: 11.205
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