Literature DB >> 29927113

Generating FN3-Based Affinity Reagents Through Phage Display.

Kevin Gorman1, Jennifer McGinnis1, Brian Kay1.   

Abstract

Antibodies are useful tools for detecting individual proteins in complex samples and for learning about their location, amount, binding partners, and function in cells. Unfortunately, generating antibodies is time consuming and laborious, and their affinity and/or specificity is often limited. This protocol offers a fast and inexpensive alternative to generate antibody surrogates through phage display of a library of fibronectin type III (FN3) monobody variants and affinity selection for binders. © 2018 by John Wiley & Sons, Inc.
Copyright © 2018 John Wiley & Sons, Inc.

Entities:  

Keywords:  affinity; immunassay; monobody; phage display; purification

Mesh：

Substances：

Year:  2018        PMID： 29927113      PMCID： PMC6020030          DOI： 10.1002/cpch.39

Source DB:  PubMed          Journal:  Curr Protoc Chem Biol        ISSN： 2160-4762


  57 in total

Review 1.  SUMO, ubiquitin's mysterious cousin.

Authors:  S Müller; C Hoege; G Pyrowolakis; S Jentsch
Journal:  Nat Rev Mol Cell Biol       Date:  2001-03       Impact factor: 94.444

2.  Efficient phage display of polypeptides fused to the carboxy-terminus of the M13 gene-3 minor coat protein.

Authors:  G Fuh; S S Sidhu
Journal:  FEBS Lett       Date:  2000-09-01       Impact factor: 4.124

Review 3.  A new generation of protein display scaffolds for molecular recognition.

Authors:  Ralf J Hosse; Achim Rothe; Barbara E Power
Journal:  Protein Sci       Date:  2006-01       Impact factor: 6.725

4.  Identification and specificity of pilus adsorption proteins of filamentous bacteriophages infecting Pseudomonas aeruginosa.

Authors:  Samantha J Holland; Carolina Sanz; Richard N Perham
Journal:  Virology       Date:  2005-11-18       Impact factor: 3.616

5.  A morphologic study of filamentous phage infection of Escherichia coli using biotinylated phages.

Authors:  Michihiro Nakamura; Kouhei Tsumoto; Izumi Kumagai; Kazunori Ishimura
Journal:  FEBS Lett       Date:  2003-02-11       Impact factor: 4.124

6.  Engineered fibronectin type III domain with a RGDWXE sequence binds with enhanced affinity and specificity to human alphavbeta3 integrin.

Authors:  Julie Richards; Michelle Miller; Johanna Abend; Akiko Koide; Shohei Koide; Stephen Dewhurst
Journal:  J Mol Biol       Date:  2003-03-07       Impact factor: 5.469

7.  Construction of proteolysis resistant human interleukin-2 by fusion to its protective single chain antibody.

Authors:  Alexander Klimka; Nelson Yu; Ezekiel Y Shami
Journal:  Cytokine       Date:  2003-06-07       Impact factor: 3.861

8.  Structural and functional analysis of phosphorylation-specific binders of the kinase ERK from designed ankyrin repeat protein libraries.

Authors:  Lutz Kummer; Petra Parizek; Peter Rube; Bastian Millgramm; Anke Prinz; Peer R E Mittl; Melanie Kaufholz; Bastian Zimmermann; Friedrich W Herberg; Andreas Plückthun
Journal:  Proc Natl Acad Sci U S A       Date:  2012-07-27       Impact factor: 11.205

9.  Streamlining the Pipeline for Generation of Recombinant Affinity Reagents by Integrating the Affinity Maturation Step.

Authors:  Renhua Huang; Kevin T Gorman; Chris R Vinci; Elena Dobrovetsky; Susanne Gräslund; Brian K Kay
Journal:  Int J Mol Sci       Date:  2015-09-30       Impact factor: 5.923

10.  Microarray profiling of phage-display selections for rapid mapping of transcription factor-DNA interactions.

Authors:  Gordon Freckleton; Soyeon I Lippman; James R Broach; Saeed Tavazoie
Journal:  PLoS Genet       Date:  2009-04-10       Impact factor: 5.917
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  1 in total

Review 1.  Development and Differentiation in Monobodies Based on the Fibronectin Type 3 Domain.

Authors:  Peter G Chandler; Ashley M Buckle
Journal:  Cells       Date:  2020-03-04       Impact factor: 6.600
  1 in total

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