Literature DB >> 2992375

Construction of a cosmid clone library of Pseudomonas syringae pv. phaseolicola and isolation of genes by functional complementation.

M Ehrenshaft, D Mills.   

Abstract

A genomic library constructed from a wild-type strain of Pseudomonas syringae pv. phaseolicola in the broad-host-range cosmid vector pVK102 was used to isolate wild-type genes by complementation of Tn5-induced auxotrophic mutants. Selection pressure was required for maintenance of the vector and members of the library in strains of P. syringae.

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Year:  1985        PMID： 2992375      PMCID： PMC238591          DOI： 10.1128/aem.50.1.169-171.1985

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  10 in total

1.  Cloning determinants of pathogenesis from Pseudomonas syringae pathovar syringae.

Authors:  F Niepold; D Anderson; D Mills
Journal:  Proc Natl Acad Sci U S A       Date:  1985-01       Impact factor: 11.205

2.  Detection and characterization of plasmids in Pseudomonas glycinea.

Authors:  M S Curiale; D Mills
Journal:  J Bacteriol       Date:  1977-07       Impact factor: 3.490

3.  Transformation and preservation of competent bacterial cells by freezing.

Authors:  D A Morrison
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

4.  Ancestral remnants in deoxyribonucleic acid from Pseudomonas and Xanthomonas.

Authors:  I W Park; J De Ley
Journal:  Antonie Van Leeuwenhoek       Date:  1967       Impact factor: 2.271

5.  Wide host range cloning vectors: a cosmid clone bank of an Agrobacterium Ti plasmid.

Authors:  V C Knauf; E W Nester
Journal:  Plasmid       Date:  1982-07       Impact factor: 3.466

6.  Integration and partial excision of a cryptic plasmid in Pseudomonas syringae pv. phaseolicola.

Authors:  M S Curiale; D Mills
Journal:  J Bacteriol       Date:  1982-11       Impact factor: 3.490

7.  Construction of a Rhizobium japonicum gene bank and use in isolation of a hydrogen uptake gene.

Authors:  M A Cantrell; R A Haugland; H J Evans
Journal:  Proc Natl Acad Sci U S A       Date:  1983-01       Impact factor: 11.205

8.  Cloned avirulence gene of Pseudomonas syringae pv. glycinea determines race-specific incompatibility on Glycine max (L.) Merr.

Authors:  B J Staskawicz; D Dahlbeck; N T Keen
Journal:  Proc Natl Acad Sci U S A       Date:  1984-10       Impact factor: 11.205

9.  Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.

Authors:  G Ditta; S Stanfield; D Corbin; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1980-12       Impact factor: 11.205

10.  Cloning of genes involved in pathogenicity of Xanthomonas campestris pv. campestris using the broad host range cosmid pLAFR1.

Authors:  M J Daniels; C E Barber; P C Turner; M K Sawczyc; R J Byrde; A H Fielding
Journal:  EMBO J       Date:  1984-12-20       Impact factor: 11.598
  10 in total
  3 in total

1.  Identification and mapping of regions that confer plasmid functions and of sites for excisive recombination of plasmid pMMC7105.

Authors:  A R Poplawsky; D Mills
Journal:  Mol Gen Genet       Date:  1987-02

2.  Construction of a stable shuttle vector for high-frequency transformation in Pseudomonas syringae pv. syringae.

Authors:  P Mukhopadhyay; M Mukhopadhyay; D Mills
Journal:  J Bacteriol       Date:  1990-01       Impact factor: 3.490

3.  Characterization of genes required for pilus expression in Pseudomonas syringae pathovar phaseolicola.

Authors:  E Roine; D N Nunn; L Paulin; M Romantschuk
Journal:  J Bacteriol       Date:  1996-01       Impact factor: 3.490
  3 in total

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