Literature DB >> 2294094

Construction of a stable shuttle vector for high-frequency transformation in Pseudomonas syringae pv. syringae.

P Mukhopadhyay1, M Mukhopadhyay, D Mills.   

Abstract

A cryptic 80.3-kilobase plasmid, pOSU900, in Pseudomonas syringae pv. syringae strain J900 could be cured by treatment with mitomycin without affecting the pathogenicity of J900 on the host, Phaseolus vulgaris L. The replication region of pOSU900 was identified, subcloned, and modified for construction of a high-copy cloning vector. This vector could be transformed into Pseudomonas strains with high efficiency (ca. 10(6) transformants per microgram of DNA) and was very stable during growth of the host bacteria in planta.

Entities:  

Mesh：

Year:  1990        PMID： 2294094      PMCID： PMC208456          DOI： 10.1128/jb.172.1.477-480.1990

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  14 in total

1.  Cloning determinants of pathogenesis from Pseudomonas syringae pathovar syringae.

Authors:  F Niepold; D Anderson; D Mills
Journal:  Proc Natl Acad Sci U S A       Date:  1985-01       Impact factor: 11.205

2.  Partitioning of the pSC101 plasmid during cell division.

Authors:  S N Cohen; C A Miller; W T Tucker; P A Meacock; P Gustafsson
Journal:  Basic Life Sci       Date:  1985

3.  Identification and cloning of genes involved in phaseolotoxin production by Pseudomonas syringae pv. "phaseolicola".

Authors:  R C Peet; P B Lindgren; D K Willis; N J Panopoulos
Journal:  J Bacteriol       Date:  1986-06       Impact factor: 3.490

4.  Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing.

Authors:  S Henikoff
Journal:  Gene       Date:  1984-06       Impact factor: 3.688

5.  Integration and partial excision of a cryptic plasmid in Pseudomonas syringae pv. phaseolicola.

Authors:  M S Curiale; D Mills
Journal:  J Bacteriol       Date:  1982-11       Impact factor: 3.490

6.  The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.

Authors:  J Vieira; J Messing
Journal:  Gene       Date:  1982-10       Impact factor: 3.688

7.  Control of plasmid replication: theoretical considerations and practical solutions.

Authors:  K Nordström
Journal:  Basic Life Sci       Date:  1985

8.  Gene cluster of Pseudomonas syringae pv. "phaseolicola" controls pathogenicity of bean plants and hypersensitivity of nonhost plants.

Authors:  P B Lindgren; R C Peet; N J Panopoulos
Journal:  J Bacteriol       Date:  1986-11       Impact factor: 3.490

9.  Cloned avirulence gene of Pseudomonas syringae pv. glycinea determines race-specific incompatibility on Glycine max (L.) Merr.

Authors:  B J Staskawicz; D Dahlbeck; N T Keen
Journal:  Proc Natl Acad Sci U S A       Date:  1984-10       Impact factor: 11.205

10.  Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.

Authors:  G Ditta; S Stanfield; D Corbin; D R Helinski
Journal:  Proc Natl Acad Sci U S A       Date:  1980-12       Impact factor: 11.205
View more
  2 in total

1.  Phylogenetic analysis of the pPT23A plasmid family of Pseudomonas syringae.

Authors:  Zhonghua Ma; James J Smith; Youfu Zhao; Robert W Jackson; Dawn L Arnold; Jesús Murillo; George W Sundin
Journal:  Appl Environ Microbiol       Date:  2006-11-17       Impact factor: 4.792

2.  Genetic and plasmid diversity within natural populations of Pseudomonas syringae with various exposures to copper and streptomycin bactericides.

Authors:  G W Sundin; D H Demezas; C L Bender
Journal:  Appl Environ Microbiol       Date:  1994-12       Impact factor: 4.792
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.