Literature DB >> 2989776

Overproduction of the EcoR V endonuclease and methylase.

L Bougueleret, M L Tenchini, J Botterman, M Zabeau.   

Abstract

Strains overproducing the EcoR V endonuclease and methylase have been obtained by inserting each of the two genes in expression vectors containing the lambda PL promoter. The methylase is overproduced to a level reaching 5-10% of the total cellular proteins, which represents a 50-100 fold increase. A 30 fold overproduction of endonuclease was achieved by randomly positioning the EndRV gene downstream of the lambda PL promoter. The situation in the endonuclease overproducing clone resembles that encountered in maxi-cells. The strains described here allowed a quick purification of both enzymes in sufficient amounts for crystallisation attempts.

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Year:  1985        PMID： 2989776      PMCID： PMC341280          DOI： 10.1093/nar/13.11.3823

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  18 in total

1.  Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.

Authors:  A C Chang; S N Cohen
Journal:  J Bacteriol       Date:  1978-06       Impact factor: 3.490

2.  Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.

Authors:  M Dagert; S D Ehrlich
Journal:  Gene       Date:  1979-05       Impact factor: 3.688

3.  Simple method for identification of plasmid-coded proteins.

Authors:  A Sancar; A M Hack; W D Rupp
Journal:  J Bacteriol       Date:  1979-01       Impact factor: 3.490

4.  Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.

Authors:  D B Clewell
Journal:  J Bacteriol       Date:  1972-05       Impact factor: 3.490

5.  Sequence analysis of the DNA encoding the Eco RI endonuclease and methylase.

Authors:  P J Greene; M Gupta; H W Boyer; W E Brown; J M Rosenberg
Journal:  J Biol Chem       Date:  1981-03-10       Impact factor: 5.157

6.  DNA sequences of structural genes for Eco RI DNA restriction and modification enzymes.

Authors:  A K Newman; R A Rubin; S H Kim; P Modrich
Journal:  J Biol Chem       Date:  1981-03-10       Impact factor: 5.157

7.  Molecular cloning of seven mouse immunoglobulin kappa chain messenger ribonucleic acids.

Authors:  N M Gough; E A Webb; S Cory; J M Adams
Journal:  Biochemistry       Date:  1980-06-10       Impact factor: 3.162

8.  Organization of transcriptional signals in plasmids pBR322 and pACYC184.

Authors:  D Stüber; H Bujard
Journal:  Proc Natl Acad Sci U S A       Date:  1981-01       Impact factor: 11.205

9.  Construction of plasmid cloning vehicles that promote gene expression from the bacteriophage lambda pL promoter.

Authors:  H U Bernard; E Remaut; M V Hershfield; H K Das; D R Helinski; C Yanofsky; N Franklin
Journal:  Gene       Date:  1979-01       Impact factor: 3.688

10.  Cloning and expression of the Pst I restriction-modification system in Escherichia coli.

Authors:  R Y Walder; J L Hartley; J E Donelson; J A Walder
Journal:  Proc Natl Acad Sci U S A       Date:  1981-03       Impact factor: 11.205
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  9 in total

Review 1.  Type II restriction endonucleases--a historical perspective and more.

Authors:  Alfred Pingoud; Geoffrey G Wilson; Wolfgang Wende
Journal:  Nucleic Acids Res       Date:  2014-05-30       Impact factor: 16.971

2.  The cloning, purification and characterization of the Eco RV modification methylase.

Authors:  V U Nwosu; B A Connolly; S E Halford; J Garnett
Journal:  Nucleic Acids Res       Date:  1988-05-11       Impact factor: 16.971

3.  Comparison of N-terminal affinity fusion domains: effect on expression level and product heterogeneity of recombinant restriction endonuclease EcoRV.

Authors:  T Oswald; W Wende; A Pingoud; U Rinas
Journal:  Appl Microbiol Biotechnol       Date:  1994-10       Impact factor: 4.813

4.  Synthesis and properties of oligodeoxynucleotides containing the analogue 2'-deoxy-4'-thiothymidine.

Authors:  E L Hancox; B A Connolly; R T Walker
Journal:  Nucleic Acids Res       Date:  1993-07-25       Impact factor: 16.971

5.  Solution parameters modulating DNA binding specificity of the restriction endonuclease EcoRV.

Authors:  Nina Y Sidorova; Shakir Muradymov; Donald C Rau
Journal:  FEBS J       Date:  2011-06-22       Impact factor: 5.542

6.  Genetic organization and molecular analysis of the EcoVIII restriction-modification system of Escherichia coli E1585-68 and its comparison with isospecific homologs.

Authors:  Iwona Mruk; Tadeusz Kaczorowski
Journal:  Appl Environ Microbiol       Date:  2003-05       Impact factor: 4.792

7.  Continuous production of restriction endonucleases: continuous two-stage cultivation with E. coli JM103; continuous cell disintegration and purification by affinity chromatography.

Authors:  H D Beer; H E Maschke; K Schügerl
Journal:  Appl Microbiol Biotechnol       Date:  1992-11       Impact factor: 4.813

8.  Overproduction and crystallization of FokI restriction endonuclease.

Authors:  K Kita; H Kotani; N Hiraoka; T Nakamura; K Yonaha
Journal:  Nucleic Acids Res       Date:  1989-11-11       Impact factor: 16.971

9.  Metal Ion Binding at the Catalytic Site Induces Widely Distributed Changes in a Sequence Specific Protein-DNA Complex.

Authors:  Kaustubh Sinha; Sahil S Sangani; Andrew D Kehr; Gordon S Rule; Linda Jen-Jacobson
Journal:  Biochemistry       Date:  2016-10-27       Impact factor: 3.162
  9 in total

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