Rekha Gautam1, Joo-Yeun Oh2, Marisa B Marques2, Richard A Dluhy1, Rakesh P Patel2. 1. Department of Pathology and Center for Free Radical Biology, University of Alabama at Birmingham, Birmingham, Alabama. 2. Department of Chemistry Pathology and Center for Free Radical Biology, University of Alabama at Birmingham, Birmingham, Alabama.
Abstract
BACKGROUND: The therapeutic efficacy and safety of stored red blood cells (RBCs) relies on minimal in-bag hemolysis. The accuracy of current methods of measuring hemolysis can suffer as a result of specimen collection and processing artefacts. OBJECTIVE: To test whether Raman spectroscopy could be used to assess hemolysis. METHODS: RBCs were stored for as long as 42 days. Raman spectra of RBCs were measured before and after washing, and hemolysis was measured in supernatant by visible spectroscopy. RESULTS: Raman spectra indicated increased concentrations of oxyhemoglobin (oxyHb) and methemoglobin (metHb), and decreased membrane fluidity with storage age. Changes in oxyHb and metHb were associated with the intraerythrocytic and extracellular fractions, respectively. Hemolysis increased in a storage age-dependent manner. Changes in Raman bands reflective of oxyHb, metHb, and RBC membranes correlated with hemolysis; the most statistically significant change was an increased intensity of metHb and decreased membrane fluidity. CONCLUSIONS: These data suggest that Raman spectroscopy may offer a new label-free modality to assess RBC hemolysis during cold storage.
BACKGROUND: The therapeutic efficacy and safety of stored red blood cells (RBCs) relies on minimal in-bag hemolysis. The accuracy of current methods of measuring hemolysis can suffer as a result of specimen collection and processing artefacts. OBJECTIVE: To test whether Raman spectroscopy could be used to assess hemolysis. METHODS: RBCs were stored for as long as 42 days. Raman spectra of RBCs were measured before and after washing, and hemolysis was measured in supernatant by visible spectroscopy. RESULTS: Raman spectra indicated increased concentrations of oxyhemoglobin (oxyHb) and methemoglobin (metHb), and decreased membrane fluidity with storage age. Changes in oxyHb and metHb were associated with the intraerythrocytic and extracellular fractions, respectively. Hemolysis increased in a storage age-dependent manner. Changes in Raman bands reflective of oxyHb, metHb, and RBC membranes correlated with hemolysis; the most statistically significant change was an increased intensity of metHb and decreased membrane fluidity. CONCLUSIONS: These data suggest that Raman spectroscopy may offer a new label-free modality to assess RBC hemolysis during cold storage.
Authors: Chenell Donadee; Nicolaas J H Raat; Tamir Kanias; Jesús Tejero; Janet S Lee; Eric E Kelley; Xuejun Zhao; Chen Liu; Hannah Reynolds; Ivan Azarov; Sheila Frizzell; E Michael Meyer; Albert D Donnenberg; Lirong Qu; Darrel Triulzi; Daniel B Kim-Shapiro; Mark T Gladwin Journal: Circulation Date: 2011-07-11 Impact factor: 29.690
Authors: James J P Alix; Maria Plesia; Sarah A Hool; Ian Coldicott; Catherine A Kendall; Pamela J Shaw Dbe; Richard J Mead; John C Day Journal: Muscle Nerve Date: 2022-07-15 Impact factor: 3.852
Authors: Eileen Edgworth; Lisa Ernst; Zoltan Czigany; Turgay Saritas; Laura Sophie Zarnitz; Marc Wiartalla; Peter Boor; Eva Miriam Buhl; Rolf Rossaint; René H Tolba; Benedict Doorschodt; Gregor Fabry; Christian Bleilevens Journal: Antioxidants (Basel) Date: 2022-07-06
Authors: Andrea M López-Canizales; Aracely Angulo-Molina; Adriana Garibay-Escobar; Erika Silva-Campa; Miguel A Mendez-Rojas; Karla Santacruz-Gómez; Mónica Acosta-Elías; Beatriz Castañeda-Medina; Diego Soto-Puebla; Osiris Álvarez-Bajo; Alexel Burgara-Estrella; Martín Pedroza-Montero Journal: Front Physiol Date: 2021-06-04 Impact factor: 4.566
Authors: Evelina I Nikelshparg; Adil A Baizhumanov; Zhanna V Bochkova; Sergey M Novikov; Dmitry I Yakubovsky; Aleksey V Arsenin; Valentyn S Volkov; Eugene A Goodilin; Anna A Semenova; Olga Sosnovtseva; Georgy V Maksimov; Nadezda A Brazhe Journal: Biosensors (Basel) Date: 2022-01-08