| Literature DB >> 29888297 |
Xian Fu1, Zachary Adams1, Julie A Maupin-Furlow1,2.
Abstract
The ubiquitin-like (Ubl) protein is widely distributed in Archaea and involved in many cellular pathways. A well-established method to reconstitute archaeal Ubl protein conjugation in vitro is important to better understand the process of archaeal Ubl protein modification. This protocol describes the in vitro reconstitution of Ubl protein modification and following analysis of this modification in Haloferax volcanii, a halophilic archaeon serving as the model organism.Entities:
Keywords: Archaea; Post-translational modification; SAMP; Ubiquitin (Ub)
Year: 2018 PMID: 29888297 PMCID: PMC5993441 DOI: 10.21769/BioProtoc.2845
Source DB: PubMed Journal: Bio Protoc ISSN: 2331-8325