| Literature DB >> 29886541 |
Reed B Wickner1, Dmitry Kryndushkin2,3, Frank Shewmaker3, Ryan McGlinchey2, Herman K Edskes2.
Abstract
We detail some of the genetic, biochemical, and physical methods useful in studying amyloids in yeast, particularly the yeast prions. These methods include cytoduction (cytoplasmic mixing), infection of cells with prion amyloids, use of green fluorescent protein fusions with amyloid-forming proteins for cytology, protein purification and amyloid formation, and electron microscopy of filaments.Entities:
Keywords: Cytoduction; Electron diffraction; Mass per length; Protein transformation
Mesh：
Substances：
Year: 2018 PMID： 29886541 PMCID： PMC7337124 DOI： 10.1007/978-1-4939-7816-8_19
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745