| Literature DB >> 2987938 |
V B Reddy, A K Beck, A J Garramone, V Vellucci, J Lustbader, E G Bernstine.
Abstract
We have inserted the cDNAs coding for both polypeptide subunits, alpha and beta, of human choriogonadotropin (hCG) into a single simian virus 40 expression vector in such a way that they replace the viral VP2 and VP1 coding sequences, respectively. Monkey cells infected with this virus and the appropriate helper virus produce dimeric hCG. hCG produced in this system was shown to chromatograph identically to standard hCG preparations on gel filtration and to be biologically active in the mouse uterine weight assay.Entities:
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Year: 1985 PMID： 2987938 PMCID： PMC397842 DOI： 10.1073/pnas.82.11.3644
Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205