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Literature DB >> 2987646

Homologous recombination of polyoma virus DNA in mouse cells.

Abstract

We have produced nonviable deletion mutants of polyoma virus in order to study homologous recombination after DNA transfection into mouse cells. The frequency of recombination was determined by the formation of infectious virus. It was dependent on the amount of DNA transfected and the size of the region of homology between the mutations. Recombination frequencies were highest when both mutated genomes were transfected in closed circular form rather than after linearization of one or both of the recombination partners. The system described may be useful for a more detailed analysis of physiological and genetic conditions influencing the frequency of homologous recombination in mouse cells as well as to study enzymes involved and intermediates produced in this process.

Entities: Disease Species

Mesh：

Substances：
DNA, Circular
DNA, Viral

Year: 1985 PMID： 2987646 DOI： 10.1007/bf00327524

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

37 in total

1. Transformation of mammalian cells with genes from procaryotes and eucaryotes.

Authors: M Wigler; R Sweet; G K Sim; B Wold; A Pellicer; E Lacy; T Maniatis; S Silverstein; R Axel
Journal: Cell Date: 1979-04 Impact factor: 41.582

2. Recombination between temperature-sensitive mutants of simian virus 40.

Authors: D R Dubbs; M Rachmeler; S Kit
Journal: Virology Date: 1974-01 Impact factor: 3.616

3. Selective extraction of polyoma DNA from infected mouse cell cultures.

Authors: B Hirt
Journal: J Mol Biol Date: 1967-06-14 Impact factor: 5.469

4. Model for homologous recombination during transfer of DNA into mouse L cells: role for DNA ends in the recombination process.

Authors: F L Lin; K Sperle; N Sternberg
Journal: Mol Cell Biol Date: 1984-06 Impact factor: 4.272

5. Mutation near the polyoma DNA replication origin permits productive infection of F9 embryonal carcinoma cells.

Authors: F K Fujimura; P L Deininger; T Friedmann; E Linney
Journal: Cell Date: 1981-03 Impact factor: 41.582

6. How damaged is the biologically active subpopulation of transfected DNA?

Authors: C T Wake; T Gudewicz; T Porter; A White; J H Wilson
Journal: Mol Cell Biol Date: 1984-03 Impact factor: 4.272

7. Amplification and excision of integrated polyoma DNA sequences require a functional origin of replication.

Authors: S Pellegrini; L Dailey; C Basilico
Journal: Cell Date: 1984-04 Impact factor: 41.582

8. Homologous recombination between transfected DNAs.

Authors: B J Pomerantz; M Naujokas; J A Hassell
Journal: Mol Cell Biol Date: 1983-09 Impact factor: 4.272

9. Integration and excision of SV40 DNA from the chromosome of a transformed cell.

Authors: M Botchan; J Stringer; T Mitchison; J Sambrook
Journal: Cell Date: 1980-05 Impact factor: 41.582

10. Novel use of synthetic oligonucleotide insertion mutants for the study of homologous recombination in mammalian cells.

Authors: G Shapira; J L Stachelek; A Letsou; L K Soodak; R M Liskay
Journal: Proc Natl Acad Sci U S A Date: 1983-08 Impact factor: 11.205

5 in total

1. Optimal replication of plasmids carrying polyomavirus origin regions requires two high-affinity binding sites for large T antigen.

Authors: I Weichselbraun; G Haider; E Wintersberger
Journal: J Virol Date: 1989-02 Impact factor: 5.103

2. Intermolecular recombination assay for mammalian cells that produces recombinants carrying both homologous and nonhomologous junctions.

Authors: S Brouillette; P Chartrand
Journal: Mol Cell Biol Date: 1987-06 Impact factor: 4.272