Literature DB >> 2986482

The basolateral membrane of rat enterocyte: its purification from brush border contamination.

M N Orsenigo, M Tosco, G Esposito, A Faelli.   

Abstract

Basolateral membranes obtained by self-orienting Percoll-gradient centrifugation were treated with 5 mM CaCl2 to minimize the cross-contamination by brush border membranes. From marker enzyme-specific activities it was calculated that in this preparation the basolateral/brush border membrane ratio was 22.6. A low L-glucose permeability across basolateral membrane vesicles together with ATP-dependent sodium uptake was observed.

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Year:  1985        PMID: 2986482     DOI: 10.1016/0003-2697(85)90156-3

Source DB:  PubMed          Journal:  Anal Biochem        ISSN: 0003-2697            Impact factor:   3.365


  11 in total

1.  Cl/HCO3 exchange in the basolateral membrane domain of rat jejunal enterocyte.

Authors:  M N Orsenigo; M Tosco; A Faelli
Journal:  J Membr Biol       Date:  1991-10       Impact factor: 1.843

2.  The GLUT5 hexose transporter is also localized to the basolateral membrane of the human jejunum.

Authors:  S J Blakemore; J C Aledo; J James; F C Campbell; J M Lucocq; H S Hundal
Journal:  Biochem J       Date:  1995-07-01       Impact factor: 3.857

3.  Basolateral Cl/HCO3 exchange in rat jejunum: the effect of sodium.

Authors:  M Tosco; M N Orsenigo; A Faelli
Journal:  J Membr Biol       Date:  1993-08       Impact factor: 1.843

4.  Identification of different laminin binding proteins in basolateral cell membranes of human colorectal carcinomas and normal colonic mucosa.

Authors:  A Stallmach; H H Riese; D Schuppan; G Bornhöft; H Stein; E O Riecken
Journal:  Gut       Date:  1991-03       Impact factor: 23.059

5.  A creatine transporter is operative at the brush border level of the rat jejunal enterocyte.

Authors:  M Tosco; A Faelli; C Sironi; G Gastaldi; M N Orsenigo
Journal:  J Membr Biol       Date:  2004-11       Impact factor: 1.843

6.  Rat jejunal basolateral membrane Cl/HCO3 exchanger is modulated by a Na-sensitive modifier site.

Authors:  M N Orsenigo; M Tosco; A Faelli
Journal:  J Membr Biol       Date:  1994-02       Impact factor: 1.843

7.  Bicarbonate and chloride transport across rat ileal basolateral membrane.

Authors:  M Tosco; M N Orsenigo; A Faelli
Journal:  Experientia       Date:  1995-08-16

8.  Glutamine transport by basolateral plasma-membrane vesicles prepared from rabbit intestine.

Authors:  S W Wilde; M S Kilberg
Journal:  Biochem J       Date:  1991-08-01       Impact factor: 3.857

9.  Systemic short-chain fatty acids rapidly alter gastrointestinal structure, function, and expression of early response genes.

Authors:  K A Tappenden; M I McBurney
Journal:  Dig Dis Sci       Date:  1998-07       Impact factor: 3.199

10.  Stimulating effect of glucocorticosteroids on intestinal fructose transport in rats is increased by feeding a saturated fatty acid diet.

Authors:  A Thiesen; M Keelan; G E Wild; M T Clandinin; A B R Thomson
Journal:  Dig Dis Sci       Date:  2003-11       Impact factor: 3.199

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