| Literature DB >> 2986482 |
M N Orsenigo, M Tosco, G Esposito, A Faelli.
Abstract
Basolateral membranes obtained by self-orienting Percoll-gradient centrifugation were treated with 5 mM CaCl2 to minimize the cross-contamination by brush border membranes. From marker enzyme-specific activities it was calculated that in this preparation the basolateral/brush border membrane ratio was 22.6. A low L-glucose permeability across basolateral membrane vesicles together with ATP-dependent sodium uptake was observed.Entities:
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Year: 1985 PMID: 2986482 DOI: 10.1016/0003-2697(85)90156-3
Source DB: PubMed Journal: Anal Biochem ISSN: 0003-2697 Impact factor: 3.365