F H Silver1,2, R G Shah3. 1. Department of Pathology and Laboratory Medicine, Robert Wood Johnson Medical School, Rutgers, The State University of New Jersey, Piscataway, NJ, USA. 2. OptoVibronex, LLC., Mt. Bethel, PA, USA. 3. Graduate Program in Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ, USA.
Abstract
BACKGROUND: The need to quantitatively identify the composition and organization of the macromolecular components of skin, skin lesions, scars, tumors, extracellular matrices (ECMs), and wound tissue has been a goal of researchers for many decades. A variety of studies have been recently reported applying optical coherence tomography (OCT) to image skin and cutaneous lesions. MATERIALS AND METHODS: This article describes the use of vibrational OCT to image and noninvasively characterize the macromolecular components of the ECM of skin. RESULTS: We report that the major macromolecular components of skin and scar can be identified noninvasively by their characteristic moduli calculated from measurements of the resonant frequency and tissue thickness. Moduli for fat (0.03 MPa), elastic tissue (0.8 MPa), skin (2 MPa), and scar (7 MPa) can be differentiated using images and measurements of the resonant frequency and the sample thickness obtained from OCT. CONCLUSIONS: Using vibrational OCT, it is possible to identify and map the location of the macromolecular components in skin and skin lesions.
BACKGROUND: The need to quantitatively identify the composition and organization of the macromolecular components of skin, skin lesions, scars, tumors, extracellular matrices (ECMs), and wound tissue has been a goal of researchers for many decades. A variety of studies have been recently reported applying optical coherence tomography (OCT) to image skin and cutaneous lesions. MATERIALS AND METHODS: This article describes the use of vibrational OCT to image and noninvasively characterize the macromolecular components of the ECM of skin. RESULTS: We report that the major macromolecular components of skin and scar can be identified noninvasively by their characteristic moduli calculated from measurements of the resonant frequency and tissue thickness. Moduli for fat (0.03 MPa), elastic tissue (0.8 MPa), skin (2 MPa), and scar (7 MPa) can be differentiated using images and measurements of the resonant frequency and the sample thickness obtained from OCT. CONCLUSIONS: Using vibrational OCT, it is possible to identify and map the location of the macromolecular components in skin and skin lesions.
Authors: Marcos A Crespo; Hiram J Jimenez; Tanmay Deshmukh; Jose S Pulido; Ahmed Saeed Saad; Frederick H Silver; Dominick A Benedetto; Christopher J Rapuano; Zeba A Syed Journal: Transl Vis Sci Technol Date: 2022-07-08 Impact factor: 3.048
Authors: Frederick H Silver; Tanmay Deshmukh; Nikita Kelkar; Kelly Ritter; Nicole Ryan; Hari Nadiminti Journal: Dermatopathology (Basel) Date: 2021-12-13