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Literature DB >> 2985975

Drosophila P element-enhanced transfection in mammalian cells.

D W Clough, H M Lepinske, R L Davidson, R V Storti.

Abstract

We constructed a gene transfer vector containing the herpes simplex virus type 1 thymidine kinase (TK) gene flanked by Drosophila P element terminal repeats (W. R. Engels, Annu. Rev. Genet. 17:315-344). This vector was introduced into mouse LTK- cells and enhanced the frequency of stable transformation to the TK+ phenotype by approximately 50-fold relative to a similar plasmid lacking the P element terminal repeats.

Entities: Gene Species

Mesh：

Substances：

Year: 1985 PMID： 2985975 PMCID： PMC366797 DOI： 10.1128/mcb.5.4.898-901.1985

Source DB: PubMed Journal: Mol Cell Biol ISSN： 0270-7306 Impact factor: 4.272

15 in total

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

2. Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells.

Authors: M Wigler; S Silverstein; L S Lee; A Pellicer; Y c Cheng; R Axel
Journal: Cell Date: 1977-05 Impact factor: 41.582

3. Genetic and physical linkage of exogenous sequences in transformed cells.

Authors: M Perucho; D Hanahan; M Wigler
Journal: Cell Date: 1980-11 Impact factor: 41.582

4. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.

Authors: J Vieira; J Messing
Journal: Gene Date: 1982-10 Impact factor: 3.688

5. Patterns of integration of DNA microinjected into cultured mammalian cells: evidence for homologous recombination between injected plasmid DNA molecules.

Authors: K R Folger; E A Wong; G Wahl; M R Capecchi
Journal: Mol Cell Biol Date: 1982-11 Impact factor: 4.272

Drosophila P element-enhanced transfection in mammalian cells.

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

2. Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells.

3. Genetic and physical linkage of exogenous sequences in transformed cells.

4. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.

5. Patterns of integration of DNA microinjected into cultured mammalian cells: evidence for homologous recombination between injected plasmid DNA molecules.

6. Transposition of cloned P elements into Drosophila germ line chromosomes.

7. Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12.

8. Construction of a mammalian transducing vector from the genome of Moloney murine leukemia virus.

9. The human beta-globin gene and a functional viral thymidine kinase gene in developing mice.

10. Nucleotide sequence of the rightward operator of phage lambda.

1. Hermes, a functional non-Drosophilid insect gene vector from Musca domestica.