Qingzhong Liu1,2, Zhaojun Zheng1,3, Wooseong Kim1, Beth Burgwyn Fuchs1, Eleftherios Mylonakis1. 1. Division of Infectious Disease, Rhode Island Hospital, Alpert Medical School of Brown University, Providence, RI 02903, USA. 2. Department of Clinical Laboratory, Shanghai General Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200080, PR China. 3. State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science & Technology, Jiangnan University, Wuxi 214112, PR China.
Abstract
AIM: l-benzyl-3-cetyl-2-methylimidazolium iodide (NH125) can inhibit Staphylococcus aureus growth. We investigated the effects of sub-MIC concentrations of NH125 on S. aureus biofilm and virulence. Methodology & results: Three strains of S. aureus were tested. Sub-lethal concentrations of NH125 repressed biofilm formation. At partial sub-MICs, NH125 downregulated the expression of most virulence, while strain-dependent effects were found in the production of α-hemolysin, δ-hemolysin, coagulase and nuclease. In Galleria mellonella model, methicillin-resistant S. aureus pre-exposed to NH125 demonstrated significantly lower killing (p = 0.032 for 1/16 and 1/8 MICs; 0.008 for 1/4 MIC; and 0.001 for 1/2 MIC). CONCLUSION: Sub-MIC concentrations of NH125 inhibited biofilm formation and virulence of S. aureus. These findings provide further support for evaluating the clinical efficacy of NH125 in staphylococcal infection.
AIM: l-benzyl-3-cetyl-2-methylimidazolium iodide (NH125) can inhibit Staphylococcus aureus growth. We investigated the effects of sub-MIC concentrations of NH125 on S. aureus biofilm and virulence. Methodology & results: Three strains of S. aureus were tested. Sub-lethal concentrations of NH125 repressed biofilm formation. At partial sub-MICs, NH125 downregulated the expression of most virulence, while strain-dependent effects were found in the production of α-hemolysin, δ-hemolysin, coagulase and nuclease. In Galleria mellonella model, methicillin-resistant S. aureus pre-exposed to NH125 demonstrated significantly lower killing (p = 0.032 for 1/16 and 1/8 MICs; 0.008 for 1/4 MIC; and 0.001 for 1/2 MIC). CONCLUSION: Sub-MIC concentrations of NH125 inhibited biofilm formation and virulence of S. aureus. These findings provide further support for evaluating the clinical efficacy of NH125 in staphylococcal infection.
Authors: Gabriel Mitchell; Myriame Lafrance; Simon Boulanger; David Lalonde Séguin; Isabelle Guay; Mariza Gattuso; Eric Marsault; Kamal Bouarab; François Malouin Journal: J Antimicrob Chemother Date: 2011-11-30 Impact factor: 5.790
Authors: Tim Verspecht; Wannes Van Holm; Nico Boon; Kristel Bernaerts; Carlo A Daep; James G Masters; Naiera Zayed; Marc Quirynen; Wim Teughels Journal: J Oral Microbiol Date: 2021-04-20 Impact factor: 5.474