| Literature DB >> 29846060 |
Bifan Chen1, Ziqing Lin2,3, Andrew J Alpert4, Cexiong Fu5, Qunying Zhang5, Wayne A Pritts5, Ying Ge1,2,3.
Abstract
Therapeutic monoclonal antibodies (mAbs) are an important class of drugs for a wide spectrum of human diseases. Liquid chromatography (LC) coupled to mass spectrometry (MS) is one of the techniques in the forefront for comprehensive characterization of analytical attributes of mAbs. Among various protein chromatography modes, hydrophobic interaction chromatography (HIC) is a popular offline nondenaturing separation technique utilized to purify and analyze mAbs, typically with the use of non-MS-compatible mobile phases. Herein we demonstrate for the first time, the application of direct HIC-MS and HIC-tandem MS (MS/MS) with electron capture dissociation (ECD) for analyzing intact mAbs on quadrupole-time-of-flight (Q-TOF) and Fourier transform ion cyclotron resonance (FTICR) mass spectrometers, respectively. Our method allows for rapid determination of relative hydrophobicity, intact masses, and glycosylation profiles of mAbs as well as sequence and structural characterization of the complementarity-determining regions in an online configuration.Entities:
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Year: 2018 PMID： 29846060 PMCID： PMC6109971 DOI： 10.1021/acs.analchem.8b01865
Source DB: PubMed Journal: Anal Chem ISSN： 0003-2700 Impact factor: 6.986