Literature DB >> 11278038

Hydrophobic interaction chromatography of proteins.

J A Queiroz1, C T Tomaz, J M Cabral.   

Abstract

In this article, an overview of hydrophobic interaction chromatography (HIC) of proteins is given. After a brief description of protein hydrophobicity and hydrophobic interactions, we present the different proposed theories for the retention mechanism of proteins in HIC. Additionally, the main parameters to consider for the optimization of fractionation processes by HIC and the stationary phases available were described. Selected examples of protein fractionation by HIC are also presented.

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Year:  2001        PMID： 11278038     DOI： 10.1016/s0168-1656(01)00237-1

Source DB:  PubMed          Journal:  J Biotechnol        ISSN： 0168-1656            Impact factor:   3.307


  30 in total

1.  Automated hydrophobic interaction chromatography column selection for use in protein purification.

Authors:  Patrick J M Murphy; Orrin J Stone; Michelle E Anderson
Journal:  J Vis Exp       Date:  2011-09-21       Impact factor: 1.355

2.  Online Hydrophobic Interaction Chromatography-Mass Spectrometry for the Analysis of Intact Monoclonal Antibodies.

Authors:  Bifan Chen; Ziqing Lin; Andrew J Alpert; Cexiong Fu; Qunying Zhang; Wayne A Pritts; Ying Ge
Journal:  Anal Chem       Date:  2018-06-04       Impact factor: 6.986

3.  Online Hydrophobic Interaction Chromatography-Mass Spectrometry for Top-Down Proteomics.

Authors:  Bifan Chen; Ying Peng; Santosh G Valeja; Lichen Xiu; Andrew J Alpert; Ying Ge
Journal:  Anal Chem       Date:  2016-01-14       Impact factor: 6.986

4.  Three dimensional liquid chromatography coupling ion exchange chromatography/hydrophobic interaction chromatography/reverse phase chromatography for effective protein separation in top-down proteomics.

Authors:  Santosh G Valeja; Lichen Xiu; Zachery R Gregorich; Huseyin Guner; Song Jin; Ying Ge
Journal:  Anal Chem       Date:  2015-04-29       Impact factor: 6.986

5.  Hydrophobic contributions to the membrane docking of synaptotagmin 7 C2A domain: mechanistic contrast between isoforms 1 and 7.

Authors:  Devin S Brandt; Matthew D Coffman; Joseph J Falke; Jefferson D Knight
Journal:  Biochemistry       Date:  2012-09-21       Impact factor: 3.162

6.  Extractive purification of recombinant thermostable lipase from fermentation broth of Escherichia coli using an aqueous polyethylene glycol impregnated resin system.

Authors:  Joo Shun Tan; Sahar Abbasiliasi; Arbakariya B Ariff; Hui Suan Ng; Mohamad Hafizi Abu Bakar; Yin Hui Chow
Journal:  3 Biotech       Date:  2018-06-09       Impact factor: 2.406

7.  Native Reversed-Phase Liquid Chromatography: A Technique for LCMS of Intact Antibody-Drug Conjugates.

Authors:  Tse-Hong Chen; Yun Yang; Zhaorui Zhang; Cexiong Fu; Qunying Zhang; Jon D Williams; Mary J Wirth
Journal:  Anal Chem       Date:  2019-02-06       Impact factor: 6.986

8.  Ion Activation Methods for Peptides and Proteins.

Authors:  Luis A Macias; Inês C Santos; Jennifer S Brodbelt
Journal:  Anal Chem       Date:  2019-11-12       Impact factor: 6.986

Review 9.  Novel Strategies to Address the Challenges in Top-Down Proteomics.

Authors:  Jake A Melby; David S Roberts; Eli J Larson; Kyle A Brown; Elizabeth F Bayne; Song Jin; Ying Ge
Journal:  J Am Soc Mass Spectrom       Date:  2021-05-13       Impact factor: 3.109

10.  Cloning, overexpression, purification, and characterization of a polyextremophilic β-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi.

Authors:  Ram Karan; Melinda D Capes; Priya DasSarma; Shiladitya DasSarma
Journal:  BMC Biotechnol       Date:  2013-01-16       Impact factor: 2.563
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