Warning: Undefined array key "mm" in /www/wwwroot/www.ai-bt.com/si.php on line 10 Deprecated: trim(): Passing null to parameter #1 ($string) of type string is deprecated in /www/wwwroot/www.ai-bt.com/si.php on line 10 Detection of Epstein-Barr virus DNA in mouthwashes by hybridization.

Literature DB >> 2984244

Detection of Epstein-Barr virus DNA in mouthwashes by hybridization.

R F Ambinder, J R Wingard, W H Burns, S D Hayward, R Saral, H R Perry, G W Santos, G S Hayward.

Abstract

An assay for the presence of Epstein-Barr virus (EBV) DNA was developed by using a cloned EBV DNA probe. After preliminary testing showed the assay to be sensitive and specific, it was applied to 135 mouthwashes from bone marrow transplant recipients, and 21 of these tests were positive. The concentration of EBV DNA in mouthwashes in some cases was as high as 10(8) genome equivalents per ml. When compared with the lymphocyte transformation assay on the same samples, the sensitivity was 75% and the specificity was 97%. In contrast to the lymphocyte transformation assay, the hybridization was semiquantitative and yielded results in 72 h. Potential applications include monitoring the effects of various interventions, such as immunosuppressive and antiviral chemotherapy, on EBV shedding.

Entities: Species

Mesh：

Substances：
DNA, Viral

Year: 1985 PMID： 2984244 PMCID： PMC271662 DOI： 10.1128/jcm.21.3.353-356.1985

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

19 in total

1. The Epstein-Barr virus.

Authors: W Henle; G Henle; E T Lennette
Journal: Sci Am Date: 1979-07 Impact factor: 2.142

2. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.

Authors: P W Rigby; M Dieckmann; C Rhodes; P Berg
Journal: J Mol Biol Date: 1977-06-15 Impact factor: 5.469

3. Studies on leukocytes growing in continuous culture derived from normal human donors.

Authors: P Gerber; J H Monroe
Journal: J Natl Cancer Inst Date: 1968-04 Impact factor: 13.506

Review 4. Rapid viral diagnosis.

Authors: D D Richman; P H Cleveland; D C Redfield; M N Oxman; G M Wahl
Journal: J Infect Dis Date: 1984-03 Impact factor: 5.226

5. Nucleic acid spot hybridization: rapid quantitative screening of lymphoid cell lines for Epstein-Barr viral DNA.

Authors: J Brandsma; G Miller
Journal: Proc Natl Acad Sci U S A Date: 1980-11 Impact factor: 11.205

6. Infectious mononucleosis. Epstein-Barr-virus shedding in saliva and the oropharynx.

Authors: J C Niederman; G Miller; H A Pearson; J S Pagano; J M Dowaliby
Journal: N Engl J Med Date: 1976-06-17 Impact factor: 91.245

7. A monoclonal immunoblastic sarcoma in donor cells bearing Epstein-Barr virus genomes following allogeneic marrow grafting for acute lymphoblastic leukemia.

Authors: W H Schubach; R Hackman; P E Neiman; G Miller; E D Thomas
Journal: Blood Date: 1982-07 Impact factor: 22.113

8. A prospective study of oropharyngeal excretion of Epstein-Barr virus in renal homograft recipients.

Authors: S N Chatterjee; R S Chang
Journal: Scand J Infect Dis Date: 1982

9. Clinical spectrum of lymphoproliferative disorders in renal transplant recipients and evidence for the role of Epstein-Barr virus.

Authors: D W Hanto; G Frizzera; D T Purtilo; K Sakamoto; J L Sullivan; A K Saemundsen; G Klein; R L Simmons; J S Najarian
Journal: Cancer Res Date: 1981-11 Impact factor: 12.701

10. Documentation of Epstein-Barr virus infection in immunodeficient patients with life-threatening lymphoproliferative diseases by Epstein-Barr virus complementary RNA/DNA and viral DNA/DNA hybridization.

Authors: A K Saemundsen; D T Purtilo; K Sakamoto; J L Sullivan; A C Synnerholm; D Hanto; R Simmons; M Anvret; R Collins; G Klein
Journal: Cancer Res Date: 1981-11 Impact factor: 12.701

9 in total

Review 1. Diagnostic deoxyribonucleic acid probes for infectious diseases.

Authors: F C Tenover
Journal: Clin Microbiol Rev Date: 1988-01 Impact factor: 26.132

2. DNA probes for clinical applications. Patents and literature.

Authors: R J Linhardt
Journal: Appl Biochem Biotechnol Date: 1986-06 Impact factor: 2.926

3. A homogeneous nucleic acid hybridization assay based on strand displacement.

Authors: C P Vary
Journal: Nucleic Acids Res Date: 1987-09-11 Impact factor: 16.971

Review 4. Advances in the use of nucleic acid probes in diagnosis of viral diseases of man. Brief review.

Authors: M Norval; R W Bingham
Journal: Arch Virol Date: 1987 Impact factor: 2.574

5. The vector homology problem in diagnostic nucleic acid hybridization of clinical specimens.

Authors: R F Ambinder; P Charache; S Staal; P Wright; M Forman; S D Hayward; G S Hayward
Journal: J Clin Microbiol Date: 1986-07 Impact factor: 5.948

6. Epstein-Barr virus gene expression in malignant lymphomas induced by experimental virus infection of cottontop tamarins.

Authors: L S Young; S Finerty; L Brooks; F Scullion; A B Rickinson; A J Morgan
Journal: J Virol Date: 1989-05 Impact factor: 5.103

7. Oral shedding of human herpesviruses in patients undergoing radiotherapy/chemotherapy treatment for head and neck squamous cell carcinoma.

Authors: Michelle Palmieri; Victor Adriano de Oliveira Martins; Laura Masami Sumita; Tania Regina Tozetto-Mendoza; Bruna Baraldi Romano; Clarisse Martins Machado; Claudio Sergio Pannuti; Thaís Bianca Brandão; Ana Carolina Prado Ribeiro; Luciana Corrêa; Paulo Henrique Braz-Silva
Journal: Clin Oral Investig Date: 2016-12-14 Impact factor: 3.573

8. The expression of novel antigens from the Epstein-Barr virus large internal repeat.

Authors: D Walls; F Gannon
Journal: EMBO J Date: 1988-04 Impact factor: 11.598

9. Oral shedding of human herpesviruses in patients undergoing radiotherapy/chemotherapy for head and neck squamous cell carcinoma is not affected by xerostomia.

Authors: Michelle Palmieri; Mariana Ornaghi; Victor Adriano de Oliveira Martins; Luciana Correa; Thais Bianca Brandao; Ana Carolina do Prado Ribeiro; Laura Masami Sumita; Tania Regina Tozetto-Mendoza; Claudio Sergio Pannuti; Paulo Henrique Braz-Silva
Journal: J Oral Microbiol Date: 2018-05-28 Impact factor: 5.474

9 in total