Literature DB >> 2984230

A method for detecting human enteroviruses in aquatic sediments.

G D Lewis, M W Loutit, F J Austin.   

Abstract

A method is described for detecting enteroviruses in both freshwater and marine sediments. Viruses were recovered from sediments by elution into 6% beef extract at pH 9.0 and concentration with polyethylene glycol 6000. The recovery efficiency ranged from 6 to 55% for marine sediments and 16 to 77% for freshwater sediments. Although the efficiency of the method was influenced by the composition and source of the sediments it was used successfully to detect viruses occurring in marine and freshwater sediments near sewer outfalls.

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Year:  1985        PMID: 2984230     DOI: 10.1016/0166-0934(85)90101-6

Source DB:  PubMed          Journal:  J Virol Methods        ISSN: 0166-0934            Impact factor:   2.014


  4 in total

1.  Development of an effective method for recovery of viral genomic RNA from environmental silty sediments for quantitative molecular detection.

Authors:  Takayuki Miura; Yoshifumi Masago; Daisuke Sano; Tatsuo Omura
Journal:  Appl Environ Microbiol       Date:  2011-04-22       Impact factor: 4.792

2.  Detection of hepatitis A virus by hybridization with single-stranded RNA probes.

Authors:  X Jiang; M K Estes; T G Metcalf
Journal:  Appl Environ Microbiol       Date:  1987-10       Impact factor: 4.792

3.  Polyethylene glycol precipitation for recovery of pathogenic viruses, including hepatitis A virus and human rotavirus, from oyster, water, and sediment samples.

Authors:  G D Lewis; T G Metcalf
Journal:  Appl Environ Microbiol       Date:  1988-08       Impact factor: 4.792

Review 4.  Abundance and Distribution of Enteric Bacteria and Viruses in Coastal and Estuarine Sediments-a Review.

Authors:  Francis Hassard; Ceri L Gwyther; Kata Farkas; Anthony Andrews; Vera Jones; Brian Cox; Howard Brett; Davey L Jones; James E McDonald; Shelagh K Malham
Journal:  Front Microbiol       Date:  2016-11-01       Impact factor: 5.640

  4 in total

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