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Literature DB >> 2981825

Enzymatic detection of phospholipid biosynthetic enzymes after electroblotting.

Abstract

The membrane-associated enzymes phosphatidylinositol synthase (CDPdiacylglycerol:myo-inositol 3-phosphatidyltransferase; EC 2.7.8.11) and phosphatidylserine synthase (CDPdiacylglycerol:L-serine O-phosphatidyltransferase; EC 2.7.8.8) from Saccharomyces cerevisiae were detected enzymatically after sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electroblotting. Enzyme activities were measured on nitrocellulose blots by using pure enzyme preparations as well as Triton X-100-solubilized membranes. Phosphatidylinositol synthase activity migrated to Mr 34,000, and phosphatidylserine synthase activity migrated to Mr 23,000.

Entities: Chemical Species

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Year: 1985 PMID： 2981825 PMCID： PMC214951 DOI： 10.1128/jb.161.2.772-774.1985

Source DB: PubMed Journal: J Bacteriol ISSN： 0021-9193 Impact factor: 3.490

12 in total

1. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

Authors: H Towbin; T Staehelin; J Gordon
Journal: Proc Natl Acad Sci U S A Date: 1979-09 Impact factor: 11.205

2. Growth and metabolism of inositol-starved Saccharomyces cerevisiae.

Authors: S A Henry; K D Atkinson; A I Kolat; M R Culbertson
Journal: J Bacteriol Date: 1977-04 Impact factor: 3.490

3. Phosphatidylserine synthesis in Saccharomyces cerevisiae. Purification and characterization of membrane-associated phosphatidylserine synthase.

Authors: M S Bae-Lee; G M Carman
Journal: J Biol Chem Date: 1984-09-10 Impact factor: 5.157

4. Solubilization of microsomal-associated phosphatidylserine synthase and phosphatidylinositol synthase from Saccharomyces cerevisiae.

Authors: G M Carman; J Matas
Journal: Can J Microbiol Date: 1981-11 Impact factor: 2.419

5. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.

Authors: W N Burnette
Journal: Anal Biochem Date: 1981-04 Impact factor: 3.365

6. Phosphatidylinositol biosynthesis in Saccharomyces cerevisiae: purification and properties of microsome-associated phosphatidylinositol synthase.

Authors: A S Fischl; G M Carman
Journal: J Bacteriol Date: 1983-04 Impact factor: 3.490

3. Identification of mitochondrial and microsomal phosphatidylserine synthase in Saccharomyces cerevisiae as the gene product of the CHO1 structural gene.

Authors: S D Kohlwein; K Kuchler; C Sperka-Gottlieb; S A Henry; F Paltauf
Journal: J Bacteriol Date: 1988-08 Impact factor: 3.490

3 in total

Enzymatic detection of phospholipid biosynthetic enzymes after electroblotting.

1. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

2. Growth and metabolism of inositol-starved Saccharomyces cerevisiae.

3. Phosphatidylserine synthesis in Saccharomyces cerevisiae. Purification and characterization of membrane-associated phosphatidylserine synthase.

4. Solubilization of microsomal-associated phosphatidylserine synthase and phosphatidylinositol synthase from Saccharomyces cerevisiae.

5. "Western blotting": electrophoretic transfer of proteins from sodium dodecyl sulfate--polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.

6. Phosphatidylinositol biosynthesis in Saccharomyces cerevisiae: purification and properties of microsome-associated phosphatidylinositol synthase.

7. Effects of inositol starvation on phospholipid and glycan syntheses in Saccharomyces cerevisiae.

8. Yeast mutants auxotrophic for choline or ethanolamine.

9. Changes in phospholipids of Saccharomyces cerevisiae associated with inositol-less death.

10. Biogenesis of mitchondria. Phospholipid synthesis in vitro by yeast mitochondrial and microsomal fractions.

1. Regulation of phosphatidylserine synthase from Saccharomyces cerevisiae by phospholipid precursors.

2. Phosphorylation of yeast phosphatidylserine synthase in vivo and in vitro by cyclic AMP-dependent protein kinase.

3. Identification of mitochondrial and microsomal phosphatidylserine synthase in Saccharomyces cerevisiae as the gene product of the CHO1 structural gene.