| Literature DB >> 2981561 |
M Köller, W Schönfeld, J Knöller, K D Bremm, W König, B Spur, A Crea, W Peters.
Abstract
The metabolism of leukotrienes (B4, C4, D4, and E4) within human plasma was studied and a simple sample preparation is presented. It was demonstrated that leukotriene E4 and leukotriene B4 were stable during incubation at 37 degrees C using the in vitro system. In contrast, leukotriene C4 was metabolized by gamma-glutamyl transpeptidase activities into leukotriene D4 which was further metabolized by dipeptidase activities of plasma into leukotriene E4. The transition state inhibitor of gamma-glutamyl transpeptidase L-serine-borate decreased the metabolism of leukotriene C4 in plasma. Dilution of plasma demonstrated that the dipeptidase was more active compared to the gamma-glutamyl transpeptidase. The metabolizing activities of plasma were functionally characterized by fractionating the plasma proteins.Entities:
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Year: 1985 PMID： 2981561 DOI： 10.1016/0005-2760(85)90260-7
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002