Yoo Na Chung1, Sejong Chun2, Minh-Trang Thi Phan3, Myung-Hyun Nam1, Byung Min Choi4, Duck Cho5, Ji Seon Choi6. 1. Department of Laboratory Medicine, Korea University Ansan Hospital, Korea University College of Medicine, Ansan, Korea. 2. Department of Laboratory Medicine, Chonnam National University Medical School & Hospital, Gwangju, Korea. 3. Samsung Bioscience Research Institute, Samsung Medical Center, Sungkyunkwan University College of Medicine, Seoul, Korea. 4. Department of Pediatrics, Korea University Ansan Hospital, Korea University College of Medicine, Ansan, Korea. 5. Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University College of Medicine, Seoul, Korea. 6. Department of Laboratory Medicine, International St. Mary's Hospital, Catholic Kwandong University College of Medicine, Incheon, Korea.
Abstract
BACKGROUND: Chimeras are composed of two or more different populations that originated from different zygotes. Blood chimerism arising from twins have been reported in the literature. Herein, we report the first blood group chimerism in triplets. METHODS: ABO blood grouping was carried out by manual tile methods (Merck Millipore, UK) and micro-column agglutination method (Bio-Rad, Cressier sur Morat, Switzerland). Flow cytometric analysis was performed with Anti-A-PE conjugated monoclonal antibodies (BD Biosciences, San Jose, CA, USA) and FACS Canto II (BD Biosciences). Molecular analysis was performed with allele-specific polymerase chain reaction (AS-PCR) and direct sequencing of the exons 6 and 7. RESULTS: Mixed-field agglutination and weak agglutination against anti-A were revealed by ABO blood grouping. Flow cytometric analysis revealed the presence of both A cells and O cells. AS-PCR and sequencing showed two neonates with chimerism, with each neonate`s genotype being A102/O01/O02. CONCLUSION: This is the first recorded case of blood chimera from a triplet in Korea. We recommend full investigation of blood group chimerism in neonates with ABO discrepancy, as blood chimerism is subject to certain caution in the clinical environment.
BACKGROUND: Chimeras are composed of two or more different populations that originated from different zygotes. Blood chimerism arising from twins have been reported in the literature. Herein, we report the first blood group chimerism in triplets. METHODS:ABO blood grouping was carried out by manual tile methods (Merck Millipore, UK) and micro-column agglutination method (Bio-Rad, Cressier sur Morat, Switzerland). Flow cytometric analysis was performed with Anti-A-PE conjugated monoclonal antibodies (BD Biosciences, San Jose, CA, USA) and FACS Canto II (BD Biosciences). Molecular analysis was performed with allele-specific polymerase chain reaction (AS-PCR) and direct sequencing of the exons 6 and 7. RESULTS: Mixed-field agglutination and weak agglutination against anti-A were revealed by ABO blood grouping. Flow cytometric analysis revealed the presence of both A cells and O cells. AS-PCR and sequencing showed two neonates with chimerism, with each neonate`s genotype being A102/O01/O02. CONCLUSION: This is the first recorded case of blood chimera from a triplet in Korea. We recommend full investigation of blood group chimerism in neonates with ABO discrepancy, as blood chimerism is subject to certain caution in the clinical environment.
Authors: Duck Cho; Sang Ku Lee; Mark Harris Yazer; Myung Geun Shin; Jong Hee Shin; Soon Pal Suh; Jeong Won Song; Mee Jeong Jeon; Ji Young Kim; Jong Tae Park; Dong Wook Ryang Journal: J Korean Med Sci Date: 2007-06 Impact factor: 2.153