Felix Streckenbach1, Ronja Klose1, Sönke Langner2, Inga Langner3, Marcus Frank4, Andreas Wree5, Anne-Marie Neumann5, Änne Glass6, Thomas Stahnke1, Rudolf F Guthoff1, Oliver Stachs1, Tobias Lindner7. 1. Department of Ophthalmology, Rostock University Medical Center, Doberaner Straße 140, 18057, Rostock, Germany. 2. Institute of Diagnostic and Interventional Radiology, Rostock University Medical Center, Ernst-Heydemann-Str. 6, 18055, Rostock, Germany. 3. Section of Hand and Functional Microsurgery, Department of Orthopedic and Trauma Surgery, University Medicine Greifswald, Ferdinand-Sauerbruch-Straße, 17475, Greifswald, Germany. 4. Medical Biology and Electron Microscopy Centre, Rostock University Medical Center, Strempelstraße 14, 18055, Rostock, Germany. 5. Institute of Anatomy, Rostock University Medical Center, Gertrudenstraße 9, 18057, Rostock, Germany. 6. Institute for Biostatistics and Informatics in Medicine and Ageing Research, Rostock University Medical Center, Ernst-Heydemann-Str. 8, 18057, Rostock, Germany. 7. Core Facility Small Animal Imaging, Rostock University Medical Center, Schillingallee 69a, 18057, Rostock, Germany. tobias.lindner@med.uni-rostock.de.
Abstract
PURPOSE: Ultrahigh-field MRI (UHF-MRI) with an in-plane spatial resolution of less than 100 μm is known as MR microscopy (MRM). MRM provides highly resolved anatomical images and allows quantitative assessment of different tissue types using diffusion-weighted imaging (DWI). The aim of the present study was to evaluate the feasibility of combined in vivo anatomical and quantitative assessment of the developing chicken eye in ovo. PROCEDURES: Thirty-eight fertilized chicken eggs were examined at 7.1 T (ClinScan, Bruker Biospin, Germany) acquiring a dataset comprising T2-weighted anatomical images, DWI, and diffusion tensor imaging. To reduce motion artifacts, the eggs were moderately cooled before and during MR imaging. Two eggs were imaged daily for the entire developmental period, and 36 eggs were examined pairwise at only one time point of the embryonic period. Development of the eye was anatomically and quantitatively assessed. RESULTS: From the D5 embryonic stage (116-124 h), MRM allowed differentiation between lens and vitreous body. The lens core and periphery were first identified at D9. DWI allowed quantification of lens maturation based on a significant decrease in apparent diffusion coefficient values and course of fractional anisotropy. Repeated moderate cooling had no influence on the development of the chicken embryo. CONCLUSIONS: MRM allows in vivo assessment of embryonic development of the chicken eye in ovo without affecting normal development. The method provides anatomical information supplemented by quantitative evaluation of lens development using DWI. With increasing availability of ultrahigh-field MR systems, this technique may provide a noninvasive complementary tool in the field of experimental ophthalmology.
PURPOSE: Ultrahigh-field MRI (UHF-MRI) with an in-plane spatial resolution of less than 100 μm is known as MR microscopy (MRM). MRM provides highly resolved anatomical images and allows quantitative assessment of different tissue types using diffusion-weighted imaging (DWI). The aim of the present study was to evaluate the feasibility of combined in vivo anatomical and quantitative assessment of the developing chicken eye in ovo. PROCEDURES: Thirty-eight fertilized chicken eggs were examined at 7.1 T (ClinScan, Bruker Biospin, Germany) acquiring a dataset comprising T2-weighted anatomical images, DWI, and diffusion tensor imaging. To reduce motion artifacts, the eggs were moderately cooled before and during MR imaging. Two eggs were imaged daily for the entire developmental period, and 36 eggs were examined pairwise at only one time point of the embryonic period. Development of the eye was anatomically and quantitatively assessed. RESULTS: From the D5 embryonic stage (116-124 h), MRM allowed differentiation between lens and vitreous body. The lens core and periphery were first identified at D9. DWI allowed quantification of lens maturation based on a significant decrease in apparent diffusion coefficient values and course of fractional anisotropy. Repeated moderate cooling had no influence on the development of the chicken embryo. CONCLUSIONS: MRM allows in vivo assessment of embryonic development of the chicken eye in ovo without affecting normal development. The method provides anatomical information supplemented by quantitative evaluation of lens development using DWI. With increasing availability of ultrahigh-field MR systems, this technique may provide a noninvasive complementary tool in the field of experimental ophthalmology.
Authors: Jianrong Xu; Zachary Delproposto; Zien Zhou; Huicong Shen; Stephanie Yang Xuan; Qing Hang Li; E Mark Haacke; Jiani Hu Journal: PLoS One Date: 2012-03-23 Impact factor: 3.240
Authors: Inga Langner; Thomas Stahnke; Oliver Stachs; Tobias Lindner; Jens-Peter Kühn; Simon Kim; Andreas Wree; Soenke Langner Journal: BMC Dev Biol Date: 2016-06-18 Impact factor: 1.978
Authors: Tobias Lindner; Ronja Klose; Felix Streckenbach; Thomas Stahnke; Stefan Hadlich; Jens-Peter Kühn; Rudolf F Guthoff; Andreas Wree; Anne-Marie Neumann; Marcus Frank; Änne Glass; Sönke Langner; Oliver Stachs Journal: Sci Rep Date: 2017-06-01 Impact factor: 4.379
Authors: Thomas Stahnke; Tobias Lindner; Rudolf Guthoff; Oliver Stachs; Andreas Wree; Sönke Langner; Thoralf Niendorf; Niels Grabow; Änne Glass; Ebba Beller; Stefan Polei Journal: Quant Imaging Med Surg Date: 2021-07