Tatiana B Feldman1,2, Marina A Yakovleva3, Andrey V Larichev4,5, Patimat M Arbukhanova6, Alexandra Sh Radchenko3, Sergey A Borzenok6, Vladimir A Kuzmin3, Mikhail A Ostrovsky7,3. 1. Department of Molecular Physiology, Biological Faculty, Lomonosov Moscow State University, Leninskie Gory 1, Moscow, Russia, 119991. feldmantb@mail.ru. 2. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Kosygin st.4, Moscow, Russia, 119334. feldmantb@mail.ru. 3. Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Kosygin st.4, Moscow, Russia, 119334. 4. The Department of Medical Physics, Faculty of Physics, Lomonosov Moscow State University, Leninskie Gory 1, Moscow, Russia, 119991. 5. Federal Scientific Research Center Crystallography and Photonics, Institute on Laser and Information Technologies of Russian Academy of Sciences, Svyatoozerskaya st. 1, Shatura, Moscow Region, Russia, 140700. 6. Sv. Fyodorov Eye Microsurgery Complex, Beskudnikovsky bld. 59a, Moscow, Russia, 127486. 7. Department of Molecular Physiology, Biological Faculty, Lomonosov Moscow State University, Leninskie Gory 1, Moscow, Russia, 119991.
Abstract
PURPOSE: The aim of this work is the determination of quantitative diagnostic criteria based on the spectral characteristics of fundus autofluorescence to detect early stages of degeneration in the retina and retinal pigment epithelium (RPE). METHODS: RPE cell suspension samples were obtained from the cadaver eyes with and without signs of age-related macular degeneration (AMD). Fluorescence analysis at an excitation wavelength of 488 nm was performed. The fluorescence lifetimes of lipofuscin-granule fluorophores were measured by counting time-correlated photon method. RESULTS: Comparative analysis of fluorescence spectra of RPE cell suspensions from the cadaver eyes with and without signs of AMD showed a significant difference in fluorescence intensity at 530-580 nm in response to fluorescence excitation at 488 nm. It was notably higher in eyes with visual pathology than in normal eyes regardless of the age of the eye donor. Measurements of fluorescence lifetimes of lipofuscin fluorophores showed that the contribution of photooxidation and photodegradation products of bisretinoids to the total fluorescence at 530-580 nm of RPE cell suspensions was greater in eyes with visual pathology than in normal eyes. CONCLUSION: Because photooxidation and photodegradation products of bisretinoids are markers of photodestructive processes, which can cause RPE cell death and initiate degenerative processes in the retina, quantitative determination of increases in these bisretinoid products in lipofuscin granules may be used to establish quantitative diagnostic criteria for degenerative processes in the retina and RPE.
PURPOSE: The aim of this work is the determination of quantitative diagnostic criteria based on the spectral characteristics of fundus autofluorescence to detect early stages of degeneration in the retina and retinal pigment epithelium (RPE). METHODS: RPE cell suspension samples were obtained from the cadaver eyes with and without signs of age-related macular degeneration (AMD). Fluorescence analysis at an excitation wavelength of 488 nm was performed. The fluorescence lifetimes of lipofuscin-granule fluorophores were measured by counting time-correlated photon method. RESULTS: Comparative analysis of fluorescence spectra of RPE cell suspensions from the cadaver eyes with and without signs of AMD showed a significant difference in fluorescence intensity at 530-580 nm in response to fluorescence excitation at 488 nm. It was notably higher in eyes with visual pathology than in normal eyes regardless of the age of the eye donor. Measurements of fluorescence lifetimes of lipofuscin fluorophores showed that the contribution of photooxidation and photodegradation products of bisretinoids to the total fluorescence at 530-580 nm of RPE cell suspensions was greater in eyes with visual pathology than in normal eyes. CONCLUSION: Because photooxidation and photodegradation products of bisretinoids are markers of photodestructive processes, which can cause RPE cell death and initiate degenerative processes in the retina, quantitative determination of increases in these bisretinoid products in lipofuscin granules may be used to establish quantitative diagnostic criteria for degenerative processes in the retina and RPE.
Authors: Sarah Warburton; Katie Southwick; Ryan M Hardman; Aaron M Secrest; Ryan K Grow; Huijun Xin; Adam T Woolley; Gregory F Burton; Craig D Thulin Journal: Mol Vis Date: 2005-12-15 Impact factor: 2.367
Authors: Yuehong Tong; Tal Ben Ami; Sungmin Hong; Rainer Heintzmann; Guido Gerig; Zsolt Ablonczy; Christine A Curcio; Thomas Ach; R Theodore Smith Journal: Retina Date: 2016-12 Impact factor: 4.256
Authors: M A Yakovleva; T B Feldman; P M Arbukhanova; S A Borzenok; V A Kuzmin; M A Ostrovsky Journal: Dokl Biochem Biophys Date: 2017-07-20 Impact factor: 0.788
Authors: Tatiana B Feldman; Marina A Yakovleva; Patimat M Arbukhanova; Sergey A Borzenok; Alexey S Kononikhin; Igor A Popov; Evgeny N Nikolaev; Mikhail A Ostrovsky Journal: Anal Bioanal Chem Date: 2014-12-04 Impact factor: 4.142
Authors: Alan D Marmorstein; Lihua Y Marmorstein; Hirokazu Sakaguchi; Joe G Hollyfield Journal: Invest Ophthalmol Vis Sci Date: 2002-07 Impact factor: 4.799
Authors: So R Kim; Young P Jang; Steffen Jockusch; Nathan E Fishkin; Nicholas J Turro; Janet R Sparrow Journal: Proc Natl Acad Sci U S A Date: 2007-11-28 Impact factor: 11.205