Literature DB >> 29779107

Stabilization of Butyrylcholinesterase by the Entrapment into the Natural Polymer-Based Gels.

V I Lonshakova-Mukina1, E N Esimbekova2,3, V A Kratasyuk1,4.   

Abstract

A new method for obtaining stable butyrylcholinesterase (BuChE) samples based on the enzyme immobilization in starch and gelatin gels followed by drying is proposed. Coimmobilization of BuChE with the thiol group indicator 5,5'-dithiobis(2-nitrobenzoic) acid did not reduce the activity of BuChE, which allowed us to simplify the procedure and reduce the time of analysis of organophosphorus pesticides. The resulting immobilized samples retained activity for at least 300 days. BuChE samples based on the starch gel showed a greater sensitivity in the determination of pesticides as compared to the samples based on the gelatin gel.

Entities:  

Mesh：

Substances：

Year:  2018        PMID： 29779107     DOI： 10.1134/S1607672918020126

Source DB:  PubMed          Journal:  Dokl Biochem Biophys        ISSN： 1607-6729            Impact factor:   0.788


  7 in total

Review 1.  Enzyme inhibition-based biosensors and biosensing systems: questionable analytical devices.

Authors:  M D Luque de Castro; M C Herrera
Journal:  Biosens Bioelectron       Date:  2003-03       Impact factor: 10.618

2.  A new and rapid colorimetric determination of acetylcholinesterase activity.

Authors:  G L ELLMAN; K D COURTNEY; V ANDRES; R M FEATHER-STONE
Journal:  Biochem Pharmacol       Date:  1961-07       Impact factor: 5.858

Review 3.  Enzyme inhibition-based biosensors for food safety and environmental monitoring.

Authors:  Aziz Amine; Hasna Mohammadi; Ilhame Bourais; Giuseppe Palleschi
Journal:  Biosens Bioelectron       Date:  2005-08-25       Impact factor: 10.618

4.  Design of multicomponent reagents for enzymatic assays.

Authors:  E N Esimbekova; V I Lonshakova-Mukina; A E Bezrukikh; V A Kratasyuk
Journal:  Dokl Biochem Biophys       Date:  2015-05-05       Impact factor: 0.788

5.  Gelatin and starch as stabilizers of the coupled enzyme system of luminous bacteria NADH:FMN-oxidoreductase-luciferase.

Authors:  Anna Bezrukikh; Elena Esimbekova; Elena Nemtseva; Valentina Kratasyuk; Osamu Shimomura
Journal:  Anal Bioanal Chem       Date:  2014-07-08       Impact factor: 4.142

Review 6.  Twenty years research in cholinesterase biosensors: from basic research to practical applications.

Authors:  Silvana Andreescu; Jean-Louis Marty
Journal:  Biomol Eng       Date:  2006-01-27

7.  Enzyme-octadecylamine Langmuir-Blodgett membranes for ENFET biosensors.

Authors:  K Wan; J M Chovelon; N Jaffrezic-Renault
Journal:  Talanta       Date:  2000-07-31       Impact factor: 6.057
  7 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.