Literature DB >> 29736601

Conformational Assessment of Adnectin and Adnectin-Drug Conjugate by Hydrogen/Deuterium Exchange Mass Spectrometry.

Richard Y-C Huang1, Steven R O'Neil2, Daša Lipovšek2, Guodong Chen3.   

Abstract

Higher-order structure (HOS) characterization of therapeutic protein-drug conjugates for comprehensive assessment of conjugation-induced protein conformational changes is an important consideration in the biopharmaceutical industry to ensure proper behavior of protein therapeutics. In this study, conformational dynamics of a small therapeutic protein, adnectin 1, together with its drug conjugate were characterized by hydrogen/deuterium exchange mass spectrometry (HDX-MS) with different spatial resolutions. Top-down HDX allows detailed assessment of the residue-level deuterium content in the payload conjugation region. HDX-MS dataset revealed the ability of peptide-based payload/linker to retain deuterium in HDX experiments. Combined results from intact, top-down, and bottom-up HDX indicated no significant conformational changes of adnectin 1 upon payload conjugation. Graphical Abstract ᅟ.

Entities:  

Keywords:  Adnectin; Adnectin-drug conjugate; HDX; Mass spectrometry

Mesh：

Substances：

Year:  2018        PMID： 29736601     DOI： 10.1007/s13361-018-1966-2

Source DB:  PubMed          Journal:  J Am Soc Mass Spectrom        ISSN： 1044-0305            Impact factor:   3.109


  31 in total

Review 1.  Strategies and challenges for the next generation of therapeutic antibodies.

Authors:  Alain Beck; Thierry Wurch; Christian Bailly; Nathalie Corvaia
Journal:  Nat Rev Immunol       Date:  2010-05       Impact factor: 53.106

2.  Semi-automated data processing of hydrogen exchange mass spectra using HX-Express.

Authors:  David D Weis; John R Engen; Ignatius J Kass
Journal:  J Am Soc Mass Spectrom       Date:  2006-08-22       Impact factor: 3.109

3.  Improved protein hydrogen/deuterium exchange mass spectrometry platform with fully automated data processing.

Authors:  Zhongqi Zhang; Aming Zhang; Gang Xiao
Journal:  Anal Chem       Date:  2012-05-15       Impact factor: 6.986

4.  Mapping the Energetic Epitope of an Antibody/Interleukin-23 Interaction with Hydrogen/Deuterium Exchange, Fast Photochemical Oxidation of Proteins Mass Spectrometry, and Alanine Shave Mutagenesis.

Authors:  Jing Li; Hui Wei; Stanley R Krystek; Derek Bond; Ty M Brender; Daniel Cohen; Jena Feiner; Nels Hamacher; Johanna Harshman; Richard Y-C Huang; Susan H Julien; Zheng Lin; Kristina Moore; Luciano Mueller; Claire Noriega; Preeti Sejwal; Paul Sheppard; Brenda Stevens; Guodong Chen; Adrienne A Tymiak; Michael L Gross; Lumelle A Schneeweis
Journal:  Anal Chem       Date:  2017-02-09       Impact factor: 6.986

5.  Fast photochemical oxidation of proteins (FPOP) maps the epitope of EGFR binding to adnectin.

Authors:  Yuetian Yan; Guodong Chen; Hui Wei; Richard Y-C Huang; Jingjie Mo; Don L Rempel; Adrienne A Tymiak; Michael L Gross
Journal:  J Am Soc Mass Spectrom       Date:  2014-09-30       Impact factor: 3.109

6.  Localized conformational interrogation of antibody and antibody-drug conjugates by site-specific carboxyl group footprinting.

Authors:  Lucy Yan Pan; Oscar Salas-Solano; John F Valliere-Douglass
Journal:  MAbs       Date:  2016-12-08       Impact factor: 5.857

7.  Hydrogen/deuterium exchange mass spectrometry and computational modeling reveal a discontinuous epitope of an antibody/TL1A Interaction.

Authors:  Richard Y-C Huang; Stanley R Krystek; Nathan Felix; Robert F Graziano; Mohan Srinivasan; Achal Pashine; Guodong Chen
Journal:  MAbs       Date:  2017-11-14       Impact factor: 5.857

8.  Effects of desialylation on human α1-acid glycoprotein-ligand interactions.

Authors:  Richard Y-C Huang; Jeffrey W Hudgens
Journal:  Biochemistry       Date:  2013-09-27       Impact factor: 3.162

9.  Assessment of physical stability of an antibody drug conjugate by higher order structure analysis: impact of thiol- maleimide chemistry.

Authors:  Jianxin Guo; Sandeep Kumar; Amarnauth Prashad; Jason Starkey; Satish K Singh
Journal:  Pharm Res       Date:  2014-01-24       Impact factor: 4.200

10.  Probing Conformational Dynamics of Tau Protein by Hydrogen/Deuterium Exchange Mass Spectrometry.

Authors:  Richard Y-C Huang; Roxana E Iacob; Sethu Sankaranarayanan; Ling Yang; Michael Ahlijanian; Li Tao; Adrienne A Tymiak; Guodong Chen
Journal:  J Am Soc Mass Spectrom       Date:  2017-10-02       Impact factor: 3.109
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  5 in total

1.  Covalent Labeling/Mass Spectrometry of Monoclonal Antibodies with Diethylpyrocarbonate: Reaction Kinetics for Ensuring Protein Structural Integrity.

Authors:  Patanachai K Limpikirati; Bo Zhao; Xiao Pan; Stephen J Eyles; Richard W Vachet
Journal:  J Am Soc Mass Spectrom       Date:  2020-04-30       Impact factor: 3.109

2.  Hydrogen-Deuterium Exchange and Hydroxyl Radical Footprinting for Mapping Hydrophobic Interactions of Human Bromodomain with a Small Molecule Inhibitor.

Authors:  Ke Sherry Li; Elizabeth T Schaper Bergman; Brett R Beno; Richard Y-C Huang; Ekaterina Deyanova; Guodong Chen; Michael L Gross
Journal:  J Am Soc Mass Spectrom       Date:  2019-11-12       Impact factor: 3.109

Review 3.  Advances in Hydrogen/Deuterium Exchange Mass Spectrometry and the Pursuit of Challenging Biological Systems.

Authors:  Ellie I James; Taylor A Murphree; Clint Vorauer; John R Engen; Miklos Guttman
Journal:  Chem Rev       Date:  2021-09-07       Impact factor: 72.087

4.  Illuminating Biological Interactions with in Vivo Protein Footprinting.

Authors:  Jessica A Espino; Lisa M Jones
Journal:  Anal Chem       Date:  2019-05-07       Impact factor: 6.986

Review 5.  Development and Differentiation in Monobodies Based on the Fibronectin Type 3 Domain.

Authors:  Peter G Chandler; Ashley M Buckle
Journal:  Cells       Date:  2020-03-04       Impact factor: 6.600
  5 in total

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