Literature DB >> 16931036

Semi-automated data processing of hydrogen exchange mass spectra using HX-Express.

David D Weis1, John R Engen, Ignatius J Kass.   

Abstract

A Microsoft Excel utility, HX-Express, that significantly accelerates the analysis of hydrogen exchange mass spectrometry data is described. HX-Express generates deuterium uptake and peak width plots from peaks in mass spectral data. Data analysis is intentionally semi-automated, requiring that the user find the peaks to be analyzed. The peaks are entered in the form of x, y lists of m/z versus intensity or can be directly imported from Waters MassLynx software. Analysis of data with HX-Express provides the same results as manual data processing but is substantially faster. In addition to speed, HX-Express provides and preserves visual and numeric displays of the analysis process for quality control.

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Year:  2006        PMID： 16931036     DOI： 10.1016/j.jasms.2006.07.025

Source DB:  PubMed          Journal:  J Am Soc Mass Spectrom        ISSN： 1044-0305            Impact factor:   3.109


  5 in total

1.  Quantitating the statistical distribution of deuterium incorporation to extend the utility of H/D exchange MS data.

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2.  Identification and characterization of EX1 kinetics in H/D exchange mass spectrometry by peak width analysis.

Authors:  David D Weis; Thomas E Wales; John R Engen; Matthew Hotchko; Lynn F Ten Eyck
Journal:  J Am Soc Mass Spectrom       Date:  2006-07-27       Impact factor: 3.109

Review 3.  Hydrogen exchange mass spectrometry for the analysis of protein dynamics.

Authors:  Thomas E Wales; John R Engen
Journal:  Mass Spectrom Rev       Date:  2006 Jan-Feb       Impact factor: 10.946

4.  Automated extraction of backbone deuteration levels from amide H/2H mass spectrometry experiments.

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Journal:  Protein Sci       Date:  2006-03       Impact factor: 6.725

5.  Hydrogen/deuterium exchange mass spectrometry for investigating protein-ligand interactions.

Authors:  Ricardo A Garcia; Dennis Pantazatos; Francisco J Villarreal
Journal:  Assay Drug Dev Technol       Date:  2004-02       Impact factor: 1.738
  5 in total
  138 in total

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2.  Hydrogen-deuterium exchange mass spectrometry reveals the interaction of Fenna-Matthews-Olson protein and chlorosome CsmA protein.

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4.  Hydrogen exchange mass spectrometry as an analytical tool for the analysis of amyloid fibrillogenesis.

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9.  Resolving isotopic fine structure to detect and quantify natural abundance- and hydrogen/deuterium exchange-derived isotopomers.

Authors:  Qian Liu; Michael L Easterling; Jeffrey N Agar
Journal:  Anal Chem       Date:  2013-12-20       Impact factor: 6.986

10.  Characterization of Aggregation Propensity of a Human Fc-Fusion Protein Therapeutic by Hydrogen/Deuterium Exchange Mass Spectrometry.

Authors:  Richard Y-C Huang; Roxana E Iacob; Stanley R Krystek; Mi Jin; Hui Wei; Li Tao; Tapan K Das; Adrienne A Tymiak; John R Engen; Guodong Chen
Journal:  J Am Soc Mass Spectrom       Date:  2016-08-15       Impact factor: 3.109
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