| Literature DB >> 29710421 |
Xiangqian Zhang1, Meng Qi2, Bo Jing2, Fuchang Yu1, Yayun Wu1, Yankai Chang1, Aiyun Zhao2, Zilin Wei2, Haiju Dong1, Longxian Zhang1.
Abstract
A total of 321 rabbit fecal samples were collected from 10 farms in the Xinjiang Uyghur Autonomous Region, China. The prevalence of Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi in the samples was 3.4% (11/321), 1.9% (6/321), and 2.8% (9/321), respectively. Small subunit ribosomal RNA (SSU rRNA) gene sequence analysis identified all 11 Cryptosporidium-positive samples as C. cuniculus. Further subtyping based on the 60-kDaglycoprotein locus (gp60) identified five of the C. cuniculus isolates as subtype VbA24. G. duodenalis genotypes were determined by multilocus sequence typing of the SSU rRNA, triosephosphate isomerase, β-giardin and glutamate dehydrogenase loci, which confirmed that six G. duodenalis isolates belonged to subtype BIV of assemblage B. Analysis of the internal transcribed spacer region, showed that five, three, and one E. bieneusi isolates belonged to genotypes J, BEB8, and Type IV, respectively. These results suggest that Cryptosporidium spp., G. duodenalis, and E. bieneusi isolates from rabbits in China have zoonotic potential.Entities:
Keywords: 60-kDa glycoprotein gene; internal transcribed spacer locus; multilocus sequence typing; rabbit; small subunit ribosomal RNA gene
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Year: 2018 PMID： 29710421 DOI： 10.1111/jeu.12629
Source DB: PubMed Journal: J Eukaryot Microbiol ISSN： 1066-5234 Impact factor: 3.346