Paweł Kwiatkowski1, Agata Pruss1, Bartłomiej Grygorcewicz2, Bartosz Wojciuk1, Barbara Dołęgowska1, Stefania Giedrys-Kalemba1, Ewa Kochan3, Monika Sienkiewicz4. 1. 1 Department of Microbiology, Immunology, and Laboratory Medicine, Pomeranian Medical University in Szczecin , Szczecin, Poland . 2. 2 Department of Immunology, Microbiology and Physiological Chemistry, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology , Szczecin, Poland . 3. 3 Pharmaceutical Biotechnology Department, Medical University of Lodz , Lodz, Poland . 4. 4 Department of Allergology and Respiratory Rehabilitation, 2nd Chair of Otolaryngology, Medical University of Lodz , Lodz, Poland .
Abstract
AIM: The aim of the study was to investigate possible synergistic effects between several selected, commercially available essential oils and gentamicin against extended-spectrum β-lactamase (ESBL)-producing and New Delhi metallo-β-lactamase-1 (NDM-1)-producing Klebsiella pneumoniae isolates. MATERIALS AND METHODS: ESBLs production was confirmed by double-disk synergy test. Isolates positive for blaNDM-1 gene were found among the tested strains. K. pneumoniae ATCC® BAA-1705™ strain was used as a control. The checkerboard method was applied to assess the synergistic and additive action of nine essential oils: caraway, fennel, peppermint, geranium, basil, clove, thyme, clary sage, and lavender, respectively, in combination with gentamicin. RESULTS: Our results indicated that peppermint oil combined with gentamicin showed synergistic activity against both control, ESBL-producing and NDM-1-producing isolates. Caraway essential oil demonstrated synergy with gentamicin toward ESBL-producing and additionally gentamicin-resistant strains. The additive effect was observed for gentamicin combined with thyme, fennel, basil, and clary sage. CONCLUSIONS: Because of their synergistic activity with gentamicin, peppermint, and caraway oils in particular, can be considered as an alternative or an addition for the control of infections with limited therapeutic options due to multidrug resistance.
AIM: The aim of the study was to investigate possible synergistic effects between several selected, commercially available essential oils and gentamicin against extended-spectrum β-lactamase (ESBL)-producing and New Delhi metallo-β-lactamase-1 (NDM-1)-producing Klebsiella pneumoniae isolates. MATERIALS AND METHODS: ESBLs production was confirmed by double-disk synergy test. Isolates positive for blaNDM-1 gene were found among the tested strains. K. pneumoniae ATCC® BAA-1705™ strain was used as a control. The checkerboard method was applied to assess the synergistic and additive action of nine essential oils: caraway, fennel, peppermint, geranium, basil, clove, thyme, clary sage, and lavender, respectively, in combination with gentamicin. RESULTS: Our results indicated that peppermint oil combined with gentamicin showed synergistic activity against both control, ESBL-producing and NDM-1-producing isolates. Caraway essential oil demonstrated synergy with gentamicin toward ESBL-producing and additionally gentamicin-resistant strains. The additive effect was observed for gentamicin combined with thyme, fennel, basil, and clary sage. CONCLUSIONS: Because of their synergistic activity with gentamicin, peppermint, and caraway oils in particular, can be considered as an alternative or an addition for the control of infections with limited therapeutic options due to multidrug resistance.
Authors: Paweł Kwiatkowski; Monika Sienkiewicz; Agata Pruss; Łukasz Łopusiewicz; Nikola Arszyńska; Iwona Wojciechowska-Koszko; Anna Kilanowicz; Barbara Kot; Barbara Dołęgowska Journal: Antibiotics (Basel) Date: 2022-01-24
Authors: Adam Junka; Anna Żywicka; Grzegorz Chodaczek; Mariusz Dziadas; Joanna Czajkowska; Anna Duda-Madej; Marzenna Bartoszewicz; Katarzyna Mikołajewicz; Grzegorz Krasowski; Patrycja Szymczyk; Karol Fijałkowski Journal: Sci Rep Date: 2019-02-04 Impact factor: 4.379