Claudia Cristina Biguetti1,2, Franco Cavalla3, Carla Roberta Tim4, Patrícia Pinto Saraiva5, Wilson Orcini6, Leandro De Andrade Holgado7, Ana Claudia Muniz Rennó8, Mariza Akemi Matsumoto9. 1. Research and Postgraduate Pro-Rectory, Universidade do Sagrado Coração - USC, Rua Irmã Arminda 10-50, Jardim Brasil Bauru, SP, 17011-160, Brazil. Claudiabiguetti@Hotmail.Com. 2. Oral Biology Doctoral's Program, Bauru School of Dentistry, São Paulo University - FOB/USP, Alameda Octávio Pinheiro Brizola 9-75, Vila Universitária, Bauru, SP, 17012-901, Brazil. Claudiabiguetti@Hotmail.Com. 3. Department of Conservative Dentistry, School of Dentistry, Universidad de Chile, Sergio Livingstone 943, Recoleta, Santiago, RM, Chile. 4. Biotechnology Doctoral's Program, São Carlos Federal University - UFSCAR, Rodovia Washington Luís, km 235, SP-310, São Carlos, SP, 13565-905, Brazil. 5. Research and Postgraduate Pro-Rectory, Universidade do Sagrado Coração - USC, Rua Irmã Arminda 10-50, Jardim Brasil Bauru, SP, 17011-160, Brazil. 6. Molecular Biology Laboratory, Research and Postgraduate Pro-Rectory, Universidade do Sagrado Coração - USC, Rua Irmã Arminda 10-50, Jardim, 17011-160, Brazil. 7. Discipline of Oral and Maxillofacial Surgery, Department of Health Sciences, Universidade Sagrado Coração - USC, Rua Irmã Arminda 10-50, Jardim Brasil Bauru, SP, 17011-160, Brazil. 8. Department of Bioscience, Federal University of São Paulo, Campus Baixada Santista - UNIFESP, Avenida Ana Costa, 95 - Vila Matias, Santos, SP, 11060-001, Brazil. 9. Discipline of Histology and Embriology, Department of Basic Sciences, São Paulo State University - FOA/UNESP, Rua Paul Harris 1100, Casa 03, Jardim Nova Iorque Araçatuba, SP, 18016-110, Brazil.
Abstract
OBJECTIVE: The aim of the study was to analyze bone matrix (BMX) organization after bone grafting and repair using a new bioactive glass-ceramic (Biosilicate®) associated or not with particulate autogenous bone graft. MATERIAL AND METHODS: Thirty rabbits underwent surgical bilateral parietal defects and divided into groups according to the materials used: (C) control-blood clot, (BG) particulate autogenous bone, (BS) bioactive glass-ceramic, and BG + BS. After 7, 14, and 30 days post-surgery, a fragment of each specimen was fixed in - 80 °C liquid nitrogen for zymographic evaluation, while the remaining was fixed in 10% formalin for histological birefringence analysis. RESULTS: The results of this study demonstrated that matrix organization in experimental groups was significantly improved compared to C considering collagenous organization. Zymographic analysis revealed pro-MMP-2, pro-MMP-9, and active (a)-MMP-2 in all groups, showing gradual decrease of total gelatinolytic activity during the periods. At day 7, BG presented more prominent gelatinolytic activity for pro-MMP-2 and 9 and a-MMP-2, when compared to the other groups. In addition, at day 7, a 53% activation ratio (active form/[active form + latent form]) was evident in C group, 33% in BS group, and 31% in BG group. CONCLUSION: In general, BS allowed the production of a BMX similar to BG, with organized collagen deposition and MMP-2 and MMP-9 disponibility, permitting satisfactory bone remodeling at the late period. CLINICAL RELEVANCE: The evaluation of new bone substitute, with favorable biological properties, opens the possibility for its use as a viable and efficient alternative to autologous bone graft.
OBJECTIVE: The aim of the study was to analyze bone matrix (BMX) organization after bone grafting and repair using a new bioactive glass-ceramic (Biosilicate®) associated or not with particulate autogenous bone graft. MATERIAL AND METHODS: Thirty rabbits underwent surgical bilateral parietal defects and divided into groups according to the materials used: (C) control-blood clot, (BG) particulate autogenous bone, (BS) bioactive glass-ceramic, and BG + BS. After 7, 14, and 30 days post-surgery, a fragment of each specimen was fixed in - 80 °C liquid nitrogen for zymographic evaluation, while the remaining was fixed in 10% formalin for histological birefringence analysis. RESULTS: The results of this study demonstrated that matrix organization in experimental groups was significantly improved compared to C considering collagenous organization. Zymographic analysis revealed pro-MMP-2, pro-MMP-9, and active (a)-MMP-2 in all groups, showing gradual decrease of total gelatinolytic activity during the periods. At day 7, BG presented more prominent gelatinolytic activity for pro-MMP-2 and 9 and a-MMP-2, when compared to the other groups. In addition, at day 7, a 53% activation ratio (active form/[active form + latent form]) was evident in C group, 33% in BS group, and 31% in BG group. CONCLUSION: In general, BS allowed the production of a BMX similar to BG, with organized collagen deposition and MMP-2 and MMP-9 disponibility, permitting satisfactory bone remodeling at the late period. CLINICAL RELEVANCE: The evaluation of new bone substitute, with favorable biological properties, opens the possibility for its use as a viable and efficient alternative to autologous bone graft.
Entities:
Keywords:
Biomaterials; Bone repair; Picrosirius red; Vitroceramic; Zymography
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