Fahimeh Aghapour1, Ali Akbar Moghadamnia1, Andrea Nicolini2, Seydeh Narges Mousavi Kani3, Ladan Barari4, Payam Morakabati4, Leyla Rezazadeh3, Sohrab Kazemi5. 1. Neuroscience Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, IR, Iran. 2. Department of Oncology, Transplantations and New Technologies in Medicine, University of Pisa, Italy. 3. Department of Pharmacology, Faculty of Medicine, Babol University of Medical Sciences, Babol, IR, Iran. 4. Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, IR, Iran. 5. Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, IR, Iran. Electronic address: kazemi.msm@gmail.com.
Abstract
BACKGROUND: Quercetin is a plant polyphenol from the flavonoid group that plays a fundamental role in controlling homeostasis due to its potent antioxidant properties. However, quercetin has extremely low water solubility, which is a major challenge in drug absorption. METHOD: In this study, we described a simple method for the synthesis of quercetin nanoparticles. The quercetin nanoparticles had an average diameter of 82 nm and prominent yellow emission under UV irradiation. Therefore, we used an in vitro model treated with quercetin and quercetin nanoparticles to investigate the effects of quercetin nanoparticles on MCF-7 breast cancer cell line. FINDING: MCF-7 cells were cultured with different concentrations (1-100 μM) of quercetin nanoparticles at the 24th, 48th and 72 nd hours, and cell cycle and apoptosis assays were detected by flow cytometry (FCM). In this study, we found that quercetin nanoparticles (1-100 μM) could significantly reduce cell vitality, growth rate and colony formation of MCF-7 cells. CONCLUSION: Quercetin nanoparticles can inhibit cell growth by blocking the cell cycle and promoting apoptosis in MCF-7 cells more than quercetin. As a result, quercetin nanoparticles may be useful therapy or prevention on breast cancer.
BACKGROUND:Quercetin is a plant polyphenol from the flavonoid group that plays a fundamental role in controlling homeostasis due to its potent antioxidant properties. However, quercetin has extremely low water solubility, which is a major challenge in drug absorption. METHOD: In this study, we described a simple method for the synthesis of quercetin nanoparticles. The quercetin nanoparticles had an average diameter of 82 nm and prominent yellow emission under UV irradiation. Therefore, we used an in vitro model treated with quercetin and quercetin nanoparticles to investigate the effects of quercetin nanoparticles on MCF-7 breast cancer cell line. FINDING: MCF-7 cells were cultured with different concentrations (1-100 μM) of quercetin nanoparticles at the 24th, 48th and 72 nd hours, and cell cycle and apoptosis assays were detected by flow cytometry (FCM). In this study, we found that quercetin nanoparticles (1-100 μM) could significantly reduce cell vitality, growth rate and colony formation of MCF-7 cells. CONCLUSION:Quercetin nanoparticles can inhibit cell growth by blocking the cell cycle and promoting apoptosis in MCF-7 cells more than quercetin. As a result, quercetin nanoparticles may be useful therapy or prevention on breast cancer.
Authors: Ahmed Qasem Ali; Mohammad Abul Farah; Faisal M Abou-Tarboush; Khalid M Al-Anazi; M Ajmal Ali; Joongku Lee; Waleed A Q Hailan; Ahmed Hossam Mahmoud Journal: Saudi J Biol Sci Date: 2018-12-30 Impact factor: 4.219
Authors: Bahare Salehi; Laura Machin; Lianet Monzote; Javad Sharifi-Rad; Shahira M Ezzat; Mohamed A Salem; Rana M Merghany; Nihal M El Mahdy; Ceyda Sibel Kılıç; Oksana Sytar; Mehdi Sharifi-Rad; Farukh Sharopov; Natália Martins; Miquel Martorell; William C Cho Journal: ACS Omega Date: 2020-05-14