Whole Genome Sequencing of NDM-1-Producing ST11 Klebsiella pneumoniae Isolated in a Private Laboratory in Greece.

The emergence and spread of NDM-1-encoding Klebsiella pneumoniae is causing worldwide concern, whereas a second epicenter of their dissemination after the Indian subcontinent is thought to be located in the Balkans. In this study, the complete genome sequencing of an NDM-1-producing ST11 K. pneumoniae isolated in a private laboratory in Greece is presented. The genome sequencing was performed on Illumina MiniSeq. Multilocus Sequence Typing was determined using a BLAST-based approach whereas antimicrobial resistance genes and plasmid replicons were identified by ResFinder and PlasmidFinder respectively. The capsular serotype was determined by the nucleotide sequence of the wzc gene. The Rapid Annotation System Technology server v2.0 was used for genome annotation. The isolate was classified to Sequence Type 11 and to the K24 capsular serotype. Its genome consisted of 5,549,974 bp with a G + C content of 57.26%. The resistome included 16 antibiotic resistance genes, 12 located in plasmids and 4 in the chromosome. The whole genome sequence of the isolate has been deposited at GenBank to serve as future reference in the study of the epidemiology and antibiotic resistance mechanisms of carbapenemase-producing Enterobacteriaceae.