Xiangjun She1, Jiaxian Guo1, Xiaoying Liu1, Hong Zhu1,2, Tong Li1, Minwen Zhou1, Fenghua Wang1,3,2, Xiaodong Sun4,5,6. 1. Department of Ophthalmology, Shanghai General Hospital (Shanghai First People's Hospital), Shanghai Jiao Tong University, School of Medicine, Shanghai, China. 2. Shanghai Engineering Center for Visual Science and Photomedicine, Shanghai, China. 3. Shanghai Key Laboratory of Fundus Diseases, Shanghai, China. 4. Department of Ophthalmology, Shanghai General Hospital (Shanghai First People's Hospital), Shanghai Jiao Tong University, School of Medicine, Shanghai, Chinaxdsun@sjtu.edu.cn. 5. Shanghai Key Laboratory of Fundus Diseases, Shanghai, Chinaxdsun@sjtu.edu.cn. 6. Shanghai Engineering Center for Visual Science and Photomedicine, Shanghai, Chinaxdsun@sjtu.edu.cn.
Abstract
PURPOSE: To evaluate the reliability of vessel density measurements in the peripapillary retina using optical coherence tomography angiography (OCT-A) and to analyze the correlation with retinal nerve fiber layer (RNFL) thickness in healthy subjects. METHOD: Thirty-five healthy volunteers were recruited in the study. The optic disc region was scanned three times with spectral-domain OCT (SD-OCT) and split-spectrum amplitude decorrelation angiography by two skilled examiners. Vessel density of the peripapillary retina was automatically calculated by the software RTVue-XR (version 2015.1.1.98). The RNFL thickness on the optic nerve head was measured by SD-OCT. The coefficient of variation (CV), coefficient of repeatability, and intraclass correlation coefficients (ICC) were calculated for intraobserver repeatability. The Bland-Altman analysis was used to determine interobserver reproducibility. Correlations between peripapillary retinal vessel density and RNFL thickness were analyzed using a multivariable linear regression. RESULTS: The mean age of the volunteers was 47.0 ± 29.7 years. The intraobserver repeatability in different sectors of the peripapillary retina was good with a high coefficient of repeatability, low CV (< 0.2%), and high ICC (0.847-0.952). The interobserver reproducibility was also good in different sectors, but should be interpreted with caution due to the difference bias caused by different observers in some quadrants. There was a significant positive correlation between vessel density and RNFL thickness; optic disc rim area and disc area were negatively related to vessel density (p = 0.008 and p = 0.001, respectively). CONCLUSION: Vessel density measurements showed good repeatability and reproducibility by OCT-A in the peripapillary retina, the vessel density was positively related to RNFL thickness and negatively related to optic disc area and rim area.
PURPOSE: To evaluate the reliability of vessel density measurements in the peripapillary retina using optical coherence tomography angiography (OCT-A) and to analyze the correlation with retinal nerve fiber layer (RNFL) thickness in healthy subjects. METHOD: Thirty-five healthy volunteers were recruited in the study. The optic disc region was scanned three times with spectral-domain OCT (SD-OCT) and split-spectrum amplitude decorrelation angiography by two skilled examiners. Vessel density of the peripapillary retina was automatically calculated by the software RTVue-XR (version 2015.1.1.98). The RNFL thickness on the optic nerve head was measured by SD-OCT. The coefficient of variation (CV), coefficient of repeatability, and intraclass correlation coefficients (ICC) were calculated for intraobserver repeatability. The Bland-Altman analysis was used to determine interobserver reproducibility. Correlations between peripapillary retinal vessel density and RNFL thickness were analyzed using a multivariable linear regression. RESULTS: The mean age of the volunteers was 47.0 ± 29.7 years. The intraobserver repeatability in different sectors of the peripapillary retina was good with a high coefficient of repeatability, low CV (< 0.2%), and high ICC (0.847-0.952). The interobserver reproducibility was also good in different sectors, but should be interpreted with caution due to the difference bias caused by different observers in some quadrants. There was a significant positive correlation between vessel density and RNFL thickness; optic disc rim area and disc area were negatively related to vessel density (p = 0.008 and p = 0.001, respectively). CONCLUSION: Vessel density measurements showed good repeatability and reproducibility by OCT-A in the peripapillary retina, the vessel density was positively related to RNFL thickness and negatively related to optic disc area and rim area.
Authors: Andrew J Nelson; Ryuna Chang; Vivian LeTran; Brian Vu; Bruce Burkemper; Zhongdi Chu; Ali Fard; Amir Kashani; Benjamin Xu; Ruikang Wang; Rohit Varma; Grace M Richter Journal: Invest Ophthalmol Vis Sci Date: 2019-08-01 Impact factor: 4.799
Authors: Jae Chang Lee; Dominic J Grisafe; Bruce Burkemper; Brenda R Chang; Xiao Zhou; Zhongdi Chu; Ali Fard; Mary Durbin; Brandon J Wong; Brian J Song; Benjamin Y Xu; Ruikang Wang; Grace M Richter Journal: Br J Ophthalmol Date: 2020-09-11 Impact factor: 4.638