| Literature DB >> 29674746 |
Megumi Shiota1, Masayuki Naya1, Takehiro Yamamoto2, Takako Hishiki2, Takeharu Tani1, Hiroyuki Takahashi3, Akiko Kubo2, Daisuke Koike4, Mai Itoh2, Mitsuyo Ohmura2, Yasuaki Kabe2, Yuki Sugiura2, Nobuyoshi Hiraoka5, Takayuki Morikawa6, Keiyo Takubo6, Kentaro Suina7, Hideaki Nagashima7, Oltea Sampetrean7, Osamu Nagano7, Hideyuki Saya7, Shogo Yamazoe1, Hiroyuki Watanabe1, Makoto Suematsu8.
Abstract
Gold deposition with diagonal angle towards boehmite-based nanostructure creates random arrays of horse-bean-shaped nanostructures named gold-nanofève (GNF). GNF generates many electromagnetic hotspots as surface-enhanced Raman spectroscopy (SERS) excitation sources, and enables large-area visualization of molecular vibration fingerprints of metabolites in human cancer xenografts in livers of immunodeficient mice with sufficient sensitivity and uniformity. Differential screening of GNF-SERS signals in tumours and those in parenchyma demarcated tumour boundaries in liver tissues. Furthermore, GNF-SERS combined with quantum chemical calculation identified cysteine-derived glutathione and hypotaurine (HT) as tumour-dominant and parenchyma-dominant metabolites, respectively. CD44 knockdown in cancer diminished glutathione, but not HT in tumours. Mechanisms whereby tumours sustained HT under CD44-knockdown conditions include upregulation of PHGDH, PSAT1 and PSPH that drove glycolysis-dependent activation of serine/glycine-cleavage systems to provide one-methyl group for HT synthesis. HT was rapidly converted into taurine in cancer cells, suggesting that HT is a robust anti-oxidant for their survival under glutathione-suppressed conditions.Entities:
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Year: 2018 PMID： 29674746 PMCID： PMC5908798 DOI： 10.1038/s41467-018-03899-1
Source DB: PubMed Journal: Nat Commun ISSN： 2041-1723 Impact factor: 14.919