Literature DB >> 2966928

A new and fast method for preparing high quality lambda DNA suitable for sequencing.

G Manfioletti1, C Schneider.   

Abstract

A method is described for the rapid purification of high quality lambda DNA. The method can be used from either liquid or plate lysates and on a small scale or a large scale. It relies on the preadsobtion of all polyanions present in the lysate to an "insoluble" anion-exchange matrix (DEAE or TEAE). Phage particles are then disrupted by combined treatment with EDTA/proteinase K and the resulting DNA is precipitated by the addition of the cationic detergent cetyl (or hexadecyl)-trimethyl ammonium bromide-CTAB ("soluble" anion-exchange matrix). The precipitated CTAB-DNA complex is then exchanged to Na-DNA and ethanol precipitated. The resultant purified DNA is suitable for enzymatic reactions and provides a high quality template for dideoxy-sequence analysis.

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Year:  1988        PMID： 2966928      PMCID： PMC336438          DOI： 10.1093/nar/16.7.2873

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  8 in total

1.  RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.

Authors:  H Lehrach; D Diamond; J M Wozney; H Boedtker
Journal:  Biochemistry       Date:  1977-10-18       Impact factor: 3.162

2.  A lambda vector for directional cDNA cloning and in vitro transcription.

Authors:  G Grimaldi; G Manfioletti; C Schneider
Journal:  Nucleic Acids Res       Date:  1987-11-25       Impact factor: 16.971

3.  A new method for purifying lambda DNA from phage lysates.

Authors:  C Helms; M Y Graham; J E Dutchik; M V Olson
Journal:  DNA       Date:  1985-02

4.  A simple and fast method for preparing single stranded DNA template suitable for sequencing.

Authors:  G Del Sal; C Schneider
Journal:  Nucleic Acids Res       Date:  1987-12-10       Impact factor: 16.971

5.  Formylatable methionine transfer RNA from Mycoplasma: purification and comparison of partial nucleotide sequences with those of other prokaryotic initiator tRNAs.

Authors:  R T Walker; U L RajBhandary
Journal:  Nucleic Acids Res       Date:  1975-01       Impact factor: 16.971

6.  Rapid isolation of high molecular weight plant DNA.

Authors:  M G Murray; W F Thompson
Journal:  Nucleic Acids Res       Date:  1980-10-10       Impact factor: 16.971

7.  USE OF ALKYLTRIMETHYLAMMONIUM BROMIDES FOR THE ISOLATION OF RIBO- AND DESOXYRIBO-NUCLEIC ACIDS.

Authors:  A S JONES
Journal:  Nature       Date:  1963-07-20       Impact factor: 49.962

8.  Microdissection and cloning of DNA from a specific region of Drosophila melanogaster polytene chromosomes.

Authors:  F Scalenghe; E Turco; J E Edström; V Pirrotta; M Melli
Journal:  Chromosoma       Date:  1981       Impact factor: 4.316
  8 in total
  40 in total

1.  Target joining of duplicated insertion sequence IS21 is assisted by IstB protein in vitro.

Authors:  S Schmid; B Berger; D Haas
Journal:  J Bacteriol       Date:  1999-04       Impact factor: 3.490

2.  Diversity of a ribonucleoprotein family in tobacco chloroplasts: two new chloroplast ribonucleoproteins and a phylogenetic tree of ten chloroplast RNA-binding domains.

Authors:  L H Ye; Y Q Li; K Fukami-Kobayashi; M Go; T Konishi; A Watanabe; M Sugiura
Journal:  Nucleic Acids Res       Date:  1991-12-11       Impact factor: 16.971

3.  Members of the zinc finger protein gene family sharing a conserved N-terminal module.

Authors:  M Rosati; M Marino; A Franzè; A Tramontano; G Grimaldi
Journal:  Nucleic Acids Res       Date:  1991-10-25       Impact factor: 16.971

4.  The Escherichia coli K-12 metJ193 allele contains a point mutation which alters the hydrophobic pocket responsible for in vitro binding of S-adenosylmethionine: effects on cell growth and induction of met regulon expression.

Authors:  C D Collier; J R Johnson
Journal:  J Bacteriol       Date:  1990-07       Impact factor: 3.490

5.  Bacteriophages of Erwinia amylovora.

Authors:  J J Gill; A M Svircev; R Smith; A J Castle
Journal:  Appl Environ Microbiol       Date:  2003-04       Impact factor: 4.792

6.  The cyclin B1 gene is actively transcribed during mitosis in HeLa cells.

Authors:  S Sciortino; A Gurtner; I Manni; G Fontemaggi; A Dey; A Sacchi; K Ozato; G Piaggio
Journal:  EMBO Rep       Date:  2001-10-17       Impact factor: 8.807

7.  Lily cofactor-independent phosphoglycerate mutase: purification, partial sequencing, and immunolocalization.

Authors:  J L Wang; L L Walling; G Y Jauh; Y Q Gu; E M Lord
Journal:  Planta       Date:  1996       Impact factor: 4.116

8.  Mouse genes coding for "zinc-finger"-containing proteins: characterization and expression in differentiated cells.

Authors:  C Passananti; A Felsani; M Caruso; P Amati
Journal:  Proc Natl Acad Sci U S A       Date:  1989-12       Impact factor: 11.205

9.  A rat brain mRNA encoding a transcriptional activator homologous to the DNA binding domain of retroviral integrases.

Authors:  A Duilio; N Zambrano; A R Mogavero; R Ammendola; F Cimino; T Russo
Journal:  Nucleic Acids Res       Date:  1991-10-11       Impact factor: 16.971

10.  Isolation and nucleotide sequence of the gene (aniA) encoding the major anaerobically induced outer membrane protein of Neisseria gonorrhoeae.

Authors:  G T Hoehn; V L Clark
Journal:  Infect Immun       Date:  1992-11       Impact factor: 3.441
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