Y Xu1,2, Y H Jia1,2, L Chen1,2, W M Huang1,2, D Q Yang1,2. 1. College of Stomatology, Chongqing Medical University, Chongqing, China. 2. Chongqing key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing, China.
Abstract
BACKGROUND: The mixed dentition is an important transition period from primary teeth to permanent teeth. However, the caries prevalence of first permanent molar in mixed dentitions was about 30%, which almost represent the caries rate of permanent teeth in this period of time. Therefore, we assessed the oral bacterial profiles in young children (age 6-8) with mixed dentition with or without first molar caries for providing the research basis of caries etiology. METHODS: We collected samples of supragingival plaque and saliva from the children living in Guizhou, a rural isolated province in China. Then, we performed DNA extraction and purification followed by 454 pyrosequencing of the V1-V3 hypervariable regions of the 16S rRNA and compared our results with those of previous research. RESULTS: (i) We analyzed 48,320 unique sequences that represented 18 phyla, 29 classes, 44 orders, 74 families, 129 genera, 15,003 species-level OUT in plaque and saliva samples; (ii) longitudinally, there was the "healthy core microbiome" between healthy deciduous dentition and early mixed dentition, for example, Neisseria, Porphyromonas, Selenomonas etc.; (iii) horizontally, there also existed the "healthy core microbiome" in early mixed dentition, for example, Neisseria, Streptococcus, Prevotella etc.; (iv) the dominant bacteria detected by Lefse in caries group including Actinomycetaceae, Streptobacillus (p < 0.05) and those in caries-free group including Gammaproteobacteria, Pasteurellaceae, Aggregatibacter, Chloroflexi, (p < 0.05). CONCLUSIONS: The oral cavity is a highly heterogeneous ecosystem with the "healthy core microbiome" in children, although microbial composition shifts along with aging. In addition, the abundance and diversity of microbiota vary between caries and caries-free groups verify the ecological plaque hypothesis.
BACKGROUND: The mixed dentition is an important transition period from primary teeth to permanent teeth. However, the caries prevalence of first permanent molar in mixed dentitions was about 30%, which almost represent the caries rate of permanent teeth in this period of time. Therefore, we assessed the oral bacterial profiles in young children (age 6-8) with mixed dentition with or without first molar caries for providing the research basis of caries etiology. METHODS: We collected samples of supragingival plaque and saliva from the children living in Guizhou, a rural isolated province in China. Then, we performed DNA extraction and purification followed by 454 pyrosequencing of the V1-V3 hypervariable regions of the 16S rRNA and compared our results with those of previous research. RESULTS: (i) We analyzed 48,320 unique sequences that represented 18 phyla, 29 classes, 44 orders, 74 families, 129 genera, 15,003 species-level OUT in plaque and saliva samples; (ii) longitudinally, there was the "healthy core microbiome" between healthy deciduous dentition and early mixed dentition, for example, Neisseria, Porphyromonas, Selenomonas etc.; (iii) horizontally, there also existed the "healthy core microbiome" in early mixed dentition, for example, Neisseria, Streptococcus, Prevotella etc.; (iv) the dominant bacteria detected by Lefse in caries group including Actinomycetaceae, Streptobacillus (p < 0.05) and those in caries-free group including Gammaproteobacteria, Pasteurellaceae, Aggregatibacter, Chloroflexi, (p < 0.05). CONCLUSIONS: The oral cavity is a highly heterogeneous ecosystem with the "healthy core microbiome" in children, although microbial composition shifts along with aging. In addition, the abundance and diversity of microbiota vary between caries and caries-free groups verify the ecological plaque hypothesis.
Authors: D Kahharova; B W Brandt; M J Buijs; M Peters; R Jackson; G Eckert; B Katz; M A Keels; S M Levy; M Fontana; E Zaura Journal: J Dent Res Date: 2019-11-26 Impact factor: 6.116
Authors: Paula Frid; Divyashri Baraniya; Josefine Halbig; Veronika Rypdal; Nils Thomas Songstad; Annika Rosèn; Johanna Rykke Berstad; Berit Flatø; Fadhl Alakwaa; Elisabeth Grut Gil; Lena Cetrelli; Tsute Chen; Nezar Noor Al-Hebshi; Ellen Nordal; Mohammed Al-Haroni Journal: Front Cell Infect Microbiol Date: 2020-11-04 Impact factor: 5.293
Authors: Deborah A Williamson; Eric P F Chow; Erica L Plummer; Kate Maddaford; Gerald L Murray; Christopher K Fairley; Shivani Pasricha; Andre Mu; Catriona S Bradshaw Journal: Microbiol Spectr Date: 2022-01-12